Gibco™ HT Supplement (100X), 50mL

Gibco™ HT Supplement (100X) is a sterile, ready-to-use liquid mixture containing 10 mM sodium hypoxanthine and 1.6 mM thymidine, specifically formulated for use in post-selection recovery and nucleoside salvage pathway studies. Designed for supplementation of cell culture media following aminopterin-based selection (e.g., HAT selection), HT Supplement enables cells to recover by bypassing temporary inhibition of de novo purine and pyrimidine synthesis.

The supplement is commonly used in hybridoma technology, mutant selection protocols, and nucleoside metabolism studies, and is essential in culture systems utilizing HAT medium where thymidine and hypoxanthine support nucleotide biosynthesis through salvage pathways.

Key Features

• Defined Composition: 10 mM sodium hypoxanthine and 1.6 mM thymidine.
• Post-Selection Support: Facilitates recovery of cells previously exposed to aminopterin.
• Sterile and Stable: Sterile-filtered, with an 18-month shelf life under proper storage.
• Convenient Format: 100X concentration allows for easy dilution and integration into standard media.
• Dual cGMP Manufacturing Sites: Made in Grand Island, NY and Paisley, Scotland, ensuring global supply chain continuity and consistent quality.

Applications

• Hybridoma Culture:
  • Used after HAT selection to support rescued hybridoma cells.
• Nucleoside Salvage Pathway Studies:
  • Investigate purine and pyrimidine metabolism.
• Mutant Cell Line Selection:
  • Rescue or support cells with impaired nucleotide synthesis pathways.
• Gene Expression & Drug Testing:
  • Evaluate drug effects on nucleotide metabolism.

Specifications

Gibco™ HT Supplement (100X) is a critical reagent for researchers working with hybridomas, nucleotide salvage pathways, or metabolic rescue systems. Its defined, sterile formulation ensures reliable performance in post-selection recovery phases and supports advanced cell culture workflows requiring nucleoside supplementation.