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Specifications:
| Application | Protein Biology, Peptide Synthesis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Amino Acid & Biochemicals | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Analytical grade | Formula | C₂₁H₂₃NO₄ |
Fmoc-Ile-OH, also known as Fmoc-L-Isoleucine, is a high-purity, N-protected amino acid derivative used in Fmoc-based solid-phase peptide synthesis (SPPS). It features an Fmoc-protected α-amino group, allowing selective deprotection during stepwise peptide elongation.
Isoleucine is a branched-chain essential amino acid that contributes to hydrophobic interactions, protein folding, peptide stability, and secondary structure formation. This makes Fmoc-Ile-OH a valuable building block for custom peptide synthesis, pharmaceutical research, proteomics, enzyme-substrate studies, and protein engineering applications.
Key Features
| Feature | Details |
|---|---|
| High Purity | ≥98.0% by titration; assay verified by HPLC |
| Fmoc Protection | Protects the α-amino group for controlled Fmoc-SPPS workflows |
| Isoleucine Building Block | Enables incorporation of isoleucine residues into synthetic peptides |
| Hydrophobic Amino Acid | Supports peptide folding, stability, and hydrophobic interactions |
| SPPS Compatible | Suitable for solid-phase peptide synthesis and peptide modification |
| Research Applications | Ideal for peptide chemistry, pharmaceutical research, proteomics, and protein engineering |
Chemical Properties & Identifiers
| Property | Value |
|---|---|
| Product Name | Fmoc-Ile-OH, ≥98.0% |
| Synonym | Fmoc-L-Isoleucine |
| CAS Number | 71989-23-6 |
| Empirical Formula | C₂₁H₂₃NO₄ |
| Molecular Weight | 353.41 g/mol |
| Beilstein Registry Number | 4716717 |
| EC Number | 276-255-9 |
| MDL Number | MFCD00037125 |
| PubChem Substance ID | 57651048 |
| Optical Activity | [α]²⁰/D -12 ± 1°; c = 1% in DMF |
| Form | Solid |
| Melting Point | 145–147 °C |
| Assay | ≥98.0% by titration; verified by HPLC |
| Storage Temperature | 2–8 °C |
| Functional Groups | Fmoc, amine, carboxylic acid |
Applications
Fmoc-Ile-OH is suitable for:
- Fmoc-based solid-phase peptide synthesis
- Incorporation of isoleucine residues into synthetic peptides
- Custom peptide synthesis and peptide modification
- Peptide-based pharmaceutical and drug discovery research
- Enzyme-substrate and inhibitor studies
- Protein engineering and structural biology research
- Proteomics and biochemical research
- Bioorganic chemistry and peptidomimetic synthesis
- Studies involving hydrophobic peptide regions and protein stability
Ordering Information
| Catalog Number | Quantity |
|---|---|
| 47628-50G-F | 50 g |
| 47628-250G-F | 250 g |
Storage & Handling
Store at 2–8 °C in a dry, tightly sealed container. Protect from moisture, oxidation, and extreme temperatures. Handle in a suitable laboratory environment using appropriate personal protective equipment, including gloves, lab coat, and safety goggles.
Research Use Statement
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
- Pack Size: 250g 50g