Invitrogen™ LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit, for 633 or 635 nm excitation

Robust Viability Dye for Red Laser Flow Cytometry – Fixation-Compatible and High Brightness

The LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit is designed to distinguish live and dead eukaryotic cells prior to formaldehyde fixation and permeabilization. It is ideal for flow cytometry workflows that require intracellular staining, as it maintains its staining pattern after fixation. Optimized for use with red laser-equipped cytometers (633 or 635 nm excitation), this amine-reactive dye provides a bright, stable, and consistent signal, enabling clear separation between viable and non-viable cells using a single fluorescent channel.

Key Features and Benefits

• Fixation-Compatible and Stable
  Covalently binds to cellular amines, preserving the staining pattern even after fixation and permeabilization.
• High Brightness for Clear Discrimination
  Bright far-red fluorescence allows clear gating of dead vs. live cells in a single detection channel, simplifying panel design.
• Red Laser Compatibility
  Excitation at 633/635 nm, with emission ~655 nm—compatible with standard APC filter sets.
• Lyophilized Format for Stability
  Freeze-dried dye in 40-test vials ensures long-term performance and minimal degradation.
• Flexible for Multiparameter Panels
  Use in multicolor panels without significant spectral overlap, maximizing detector flexibility.

How It Works

• Dead cells: Dye penetrates compromised membranes and binds intracellular and surface amines, generating intense far-red fluorescence.
• Live cells: Dye binds only surface amines, resulting in significantly lower fluorescence.
• This generates a >50-fold difference in signal between live and dead cells, allowing for accurate viability gating.

Applications

• Live/dead cell discrimination in flow cytometry
• Viability staining prior to intracellular cytokine or transcription factor analysis
• Dead cell exclusion in immunophenotyping and multicolor panels
• Sample quality control in immunology and cancer research workflows

Technical Specifications

Kit Contents

• 5 vials of LIVE/DEAD™ Fixable Far Red dye (lyophilized)
• 1 vial (500 µL) of DMSO for reconstitution

Conclusion

The LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit is a powerful and reliable solution for live/dead discrimination in fixation-sensitive, high-parameter flow cytometry panels. With robust stability, strong signal, and red-laser compatibility, it’s ideal for immunophenotyping, cell sorting, and intracellular staining workflows.