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Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
For Intracellular Staining of Transcription Factors, Cytokines, and Nuclear Proteins in Flow Cytometry
The Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent is a specialized reagent system optimized for the intracellular detection of transcription factors (such as Foxp3, Ki-67) and other nuclear proteins by flow cytometry. This two-component kit enables reliable fixation and permeabilization of cells, facilitating high-quality antibody staining of intracellular targets.
Designed for flexibility, this product is a part of the modular Foxp3 / Transcription Factor Staining Buffer System and is intended for use in combination with Permeabilization Buffer (10X) (Cat. No. 00-8333), or as part of the complete staining buffer set (Cat. No. 00-5523).
Key Features and Benefits
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Optimized for Nuclear Protein Staining
Ideal for transcription factors such as Foxp3, Ki-67, and nuclear-localized cytokines and chemokines. -
Validated for Flow Cytometry
Enables consistent and reproducible intracellular staining results following surface marker staining. -
Flexible, Modular Use
Can be used independently with compatible permeabilization buffer or as part of a complete intracellular staining workflow. -
High Compatibility
Compatible with fluorochrome-conjugated antibodies, including tandem dyes, for multicolor flow cytometry.
Applications
- Intracellular staining for transcription factors and nuclear proteins
- Flow cytometric analysis of Tregs and proliferating cells
- Staining of cytokines/chemokines after cell stimulation
- Foxp3 expression analysis in regulatory T cells
Technical Specifications
| Property | Details |
|---|---|
| Catalog Number | 00-5521-00 |
| Product Name | Foxp3 / Transcription Factor Fixation/Permeabilization Kit |
| Components Included | Fixation/Permeabilization Concentrate and Diluent |
| Required Additional Reagent | Permeabilization Buffer (10X), Cat. No. 00-8333 |
| Compatible Product Set | Foxp3 / Transcription Factor Staining Buffer Set, Cat. No. 00-5523 |
| Applications | Intracellular staining, Flow cytometry |
| Reagent Type | Fixation Reagent, Permeabilization Reagent |
| Unit Size | 1 Kit |
| Storage Conditions | Store as directed on label; avoid repeated freeze-thaw cycles |
| Shipping Conditions | Ambient |
| Product Line | eBioscience™ |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent provides a reliable, validated method for preparing cells for intracellular staining of key transcription factors and nuclear proteins. Essential for flow cytometric analysis of regulatory T cells (Tregs) and cell proliferation markers, this reagent system is a must-have for immunology and cell biology researchers.