Invitrogen™ LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit, for 633 or 635 nm excitation

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Specifications:
Application Flow Cytometry, Cell Analysis
Storage Temperature -20°C
Product Type Flow Cytometry Reagents Forms Lyophilized
Product Brand Thermo Fisher Scientific™, Invitrogen
Product Grade Molecular Biology

Robust Viability Dye for Red Laser Flow Cytometry – Fixation-Compatible and High Brightness

The LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit is designed to distinguish live and dead eukaryotic cells prior to formaldehyde fixation and permeabilization. It is ideal for flow cytometry workflows that require intracellular staining, as it maintains its staining pattern after fixation. Optimized for use with red laser-equipped cytometers (633 or 635 nm excitation), this amine-reactive dye provides a bright, stable, and consistent signal, enabling clear separation between viable and non-viable cells using a single fluorescent channel.

Key Features and Benefits

  • Fixation-Compatible and Stable
    Covalently binds to cellular amines, preserving the staining pattern even after fixation and permeabilization.
  • High Brightness for Clear Discrimination
    Bright far-red fluorescence allows clear gating of dead vs. live cells in a single detection channel, simplifying panel design.
  • Red Laser Compatibility
    Excitation at 633/635 nm, with emission ~655 nm—compatible with standard APC filter sets.
  • Lyophilized Format for Stability
    Freeze-dried dye in 40-test vials ensures long-term performance and minimal degradation.
  • Flexible for Multiparameter Panels
    Use in multicolor panels without significant spectral overlap, maximizing detector flexibility.

How It Works

  • Dead cells: Dye penetrates compromised membranes and binds intracellular and surface amines, generating intense far-red fluorescence.
  • Live cells: Dye binds only surface amines, resulting in significantly lower fluorescence.
  • This generates a >50-fold difference in signal between live and dead cells, allowing for accurate viability gating.

Applications

  • Live/dead cell discrimination in flow cytometry
  • Viability staining prior to intracellular cytokine or transcription factor analysis
  • Dead cell exclusion in immunophenotyping and multicolor panels
  • Sample quality control in immunology and cancer research workflows

Technical Specifications

PropertyDetails
Product NameLIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit
Catalog NumbersL34973 (80 assays), L10120 (200 assays), L34974 (400 assays)
Excitation / Emission633–635 nm / ~655 nm
ColorFar Red
FormLyophilized solid + 500 µL DMSO
Detection MethodFluorescence
Cell PermeabilityImpermeant
Storage Conditions–20°C, protect from light
Shipping ConditionsRoom temperature
SolubilityDMSO
Instrument CompatibilityFlow cytometer with red laser
Product LineLIVE/DEAD™
Unit SizeEach (200 assays)
Intended UseFor Research Use Only. Not for use in diagnostic procedures.

Kit Contents

  • 5 vials of LIVE/DEAD™ Fixable Far Red dye (lyophilized)
  • 1 vial (500 µL) of DMSO for reconstitution

Conclusion

The LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit is a powerful and reliable solution for live/dead discrimination in fixation-sensitive, high-parameter flow cytometry panels. With robust stability, strong signal, and red-laser compatibility, it’s ideal for immunophenotyping, cell sorting, and intracellular staining workflows.

  • Pack Size:   for 80 Assays for 400 Assays for 200 Assays

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