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Thermo Scientific™ DH10B Chemically Competent E. coli Cells, High Efficiency, 10 x 100 μL
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CAS Number :
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Specifications:
| Application | Cloning | ||
| Storage Temperature | –80°C | ||
| Product Type | cells | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific DH10B Competent Cells are high-efficiency, chemically competent E. coli cells, specifically designed for DNA cloning applications. These cells are optimized for cloning prokaryotic and eukaryotic genomic DNA, including methylcytosine, 5-hydroxymethylcytosine, and methyladenine. With high transformation efficiency and the ability to generate cDNA libraries or construct gene banks, DH10B Competent Cells are the ideal choice for researchers involved in cloning large plasmids and bacterial artificial chromosomes (BACs). These cells also enable blue/white colony screening for easy identification of successful clones.
Key Features & Benefits:
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High Transformation Efficiency:
- >1x10⁹ cfu/μg of pUC19 DNA, ensuring excellent results in cloning experiments.
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Ideal for Various Cloning Applications:
- Suitable for cloning both prokaryotic and eukaryotic DNA.
- Supports the cloning of large plasmids and BACs.
- Optimized for cDNA library generation using plasmid-derived vectors.
- Enables site-directed mutagenesis for genetic manipulation.
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Blue/White Colony Screening:
- The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, facilitating easy blue/white colony screening using Bluo-Gal or X-Gal on bacterial agar plates.
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Two Reactions per Vial:
- The packaging includes two reactions per vial, making it more convenient for high-throughput or routine cloning.
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S.O.C. Outgrowth Medium Included:
- The kit includes S.O.C. outgrowth medium, which is essential for enhancing transformation efficiency after the competent cells have been exposed to DNA.
Applications:
- DNA Cloning: Efficient for cloning both prokaryotic and eukaryotic DNA.
- cDNA Library Construction: Ideal for creating cDNA libraries using plasmid vectors.
- Site-Directed Mutagenesis: Useful for introducing specific mutations into DNA sequences.
- Cloning Large Plasmids and BACs: Supports the cloning of large DNA fragments, including bacterial artificial chromosomes.
Genotype:
- F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG
Specifications:
| Feature | Details |
|---|---|
| Product Type | Competent Cell |
| Efficiency | High Efficiency (>1x10⁹ cfu/μg pUC19 DNA) |
| Cell Type | Chemically Competent |
| Libraries | cDNA |
| Cloning Methylated DNA | Yes |
| Blue/White Screening | Yes |
| High-throughput Compatibility | Not High-throughput Compatible |
| Propagating ccdB Vectors | Not for ccdB vector propagation |
| Shipping Condition | Dry Ice |
| Species | E. coli |
| Format | Tube |
| For Use With (Application) | Cloning |
| Quantity | 10 x 100 μL |
Contents:
- 10 x DH10B Competent Cells (100 μL each)
- pUC19 DNA (50 μL, 10 pg/μL)
- S.O.C. Medium (5 mL)
Storage:
- Store at -80°C for long-term storage.
Thermo Scientific DH10B Competent Cells are optimized for high-efficiency DNA cloning, making them an excellent choice for routine cloning, site-directed mutagenesis, and cDNA library construction. With high transformation efficiency, blue/white colony screening, and easy handling, these cells offer the reliability and performance needed for various cloning applications.