Invitrogen™ Collibri™ ES DNA Library Prep Kit for Illumina Systems with CD Indexes

The Invitrogen™ Collibri™ ES DNA Library Prep Kit for Illumina Systems with CD Indexes is a complete, high-performance solution for preparing indexed DNA libraries for next-generation sequencing (NGS) on Illumina platforms. Designed for speed, flexibility, and reliability, the kit enables efficient library preparation from a wide range of DNA inputs (1–500 ng), including intact genomic DNA and amplicons greater than 300 bp from any species.

This complete kit includes enzymatic fragmentation, library preparation reagents, cleanup modules, and CD (Combinatorial Dual) indexes, allowing immediate generation of multiplexed sequencing-ready libraries without the need for additional indexing components. The streamlined workflow can be completed in approximately three hours using PCR amplification, while PCR-free workflows are supported for inputs of ≥100 ng DNA, improving data accuracy and reducing amplification bias.

The advanced enzymatic shearing (ES) technology ensures consistent fragmentation and high library yield, making it suitable for high-throughput sequencing applications and multiple sequencing runs on Illumina systems.

Key Features

Complete Library Preparation Kit with CD Indexes

• Includes enzymatic fragmentation, cleanup reagents, and indexed adapters
• Enables multiplex sequencing with combinatorial dual indexing
• Ready-to-use solution for rapid implementation in sequencing workflows

Fast and Efficient Workflow

• Complete DNA library preparation in approximately 3 hours
• Reduced hands-on time improves laboratory efficiency
• Supports both PCR and PCR-free workflows

Wide Input Range and Flexible Sample Compatibility

• Compatible with DNA inputs from 1 ng to 500 ng
• Supports genomic DNA and amplicons >300 bp
• Suitable for DNA samples from any species

Advanced Enzymatic Fragmentation Technology

• Controlled enzymatic shearing ensures uniform DNA fragmentation
• Provides consistent and high library yield
• Enables reliable sequencing performance

Integrated Visual Workflow Feedback

• Inert dyes provide visual confirmation of workflow progress
• Reduces preparation errors and improves reliability

Automation and High-Throughput Ready

• Compatible with automated liquid handling systems
• Supports miniaturized workflows for reduced reagent cost
• Ideal for high-throughput sequencing laboratories

Applications

The Collibri ES DNA Library Prep Kit is optimized for a wide range of genomics and sequencing applications, including:

• Whole genome sequencing (WGS)
• Genome resequencing
• De novo genome assembly
• Microbial genome sequencing
• Human genomics research
• Agricultural and environmental genomics
• Public health and pathogen surveillance
• Academic and biotechnology research

Illumina Instrument Compatibility

Fully compatible with Illumina sequencing platforms, including:

• NovaSeq™ 6000
• NextSeq™ 550
• MiSeq™
• iSeq™
• HiSeq™ X, 2500, 3000, and 4000

Kit Contents and Storage

Ships on dry ice to maintain reagent integrity.

Technical Specifications

Advantages for Sequencing Laboratories

• Complete library preparation kit with integrated indexing
• Fast and efficient workflow for improved productivity
• High library yield and sequencing consistency
• Compatible with Illumina NovaSeq, NextSeq, MiSeq, and HiSeq systems
• Suitable for automation and high-throughput environments
• Reliable and reproducible results across diverse sample types

The Invitrogen™ Collibri™ ES DNA Library Prep Kit for Illumina Systems with CD Indexes provides a comprehensive, efficient, and flexible solution for preparing high-quality, indexed DNA libraries for Illumina sequencing. Its enzymatic fragmentation technology, integrated indexing capability, automation compatibility, and rapid workflow make it an ideal choice for genomics laboratories, sequencing facilities, research institutions, and biotechnology organizations requiring reliable and scalable NGS library preparation.