Invitrogen™ Collibri™ ES DNA Library Prep Core Kit for Illumina™ Systems

The Invitrogen™ Collibri™ ES DNA Library Prep Core Kit for Illumina Systems is an advanced enzymatic DNA library preparation solution designed to enable fast, reliable, and high-yield preparation of sequencing-ready DNA libraries for next-generation sequencing (NGS). The kit supports a wide DNA input range (1–500 ng) and is compatible with intact genomic DNA or amplicons larger than 300 bp, making it ideal for diverse genomics applications.

Powered by highly efficient enzymatic fragmentation (ES), the Collibri system provides consistent library yields while preserving DNA integrity. The streamlined workflow enables complete library preparation in approximately three hours with PCR amplification, and also supports PCR-free workflows for inputs ≥100 ng, improving sequencing accuracy and reducing bias.

The Core Kit format provides enzymatic modification and cleanup modules, allowing users flexibility to pair with their preferred indexed adapters for customized sequencing workflows on Illumina platforms including MiSeq™, NextSeq™, and NovaSeq™ systems.

Key Features

Fast and Efficient Workflow

• Complete DNA library preparation in approximately 3 hours with PCR workflow
• Rapid and streamlined process reduces hands-on time and accelerates sequencing turnaround

Wide DNA Input Compatibility

• Supports input DNA amounts from 1 ng to 500 ng
• Compatible with genomic DNA and amplicons greater than 300 bp
• PCR-free workflow option available for inputs ≥100 ng

Advanced Enzymatic Fragmentation Technology

• Enzymatic shearing ensures controlled, uniform DNA fragmentation
• Provides high library yield suitable for quantification and multiple sequencing runs

Visual Workflow Monitoring

• Integrated inert dyes provide visual feedback during library preparation steps
• Reduces errors and improves workflow reliability

Automation and High-Throughput Ready

• Fully compatible with liquid handling automation platforms
• Supports miniaturization workflows to reduce reagent consumption and cost
• Ideal for high-throughput sequencing laboratories

Flexible Core Kit Configuration

• Core Kit allows integration with selected indexed adapters
• Enables customized multiplexing strategies

Applications

The Collibri ES DNA Library Prep Core Kit is optimized for a wide range of genomic sequencing applications, including:

• Whole genome sequencing (WGS)
• Genome resequencing
• De novo genome assembly
• DNA fragment sequencing
• Population genomics studies
• Microbial genomics
• Cancer genomics research
• Agricultural genomics
• Public health genomics surveillance

Compatibility

Fully compatible with Illumina sequencing platforms, including:

• Illumina NovaSeq™ 6000
• Illumina NextSeq™ 550
• Illumina MiSeq™
• Illumina iSeq™
• Illumina HiSeq™ X, 2500, 3000, and 4000

Kit Contents and Storage

The Core Kit contains enzymatic fragmentation and cleanup modules required for library preparation.

Technical Specifications

Advantages for NGS Laboratories

• High-efficiency enzymatic fragmentation with excellent library yield
• Reduced workflow time compared to mechanical fragmentation methods
• Improved reproducibility and sequencing consistency
• Compatible with high-throughput automation systems
• Ideal for modern genomics laboratories, sequencing facilities, and research institutions

The Invitrogen™ Collibri™ ES DNA Library Prep Core Kit provides a robust, efficient, and flexible solution for preparing high-quality DNA libraries for Illumina sequencing platforms. Its advanced enzymatic fragmentation technology, rapid workflow, automation compatibility, and broad input flexibility make it an excellent choice for genomics research laboratories, sequencing core facilities, and biotechnology institutions seeking reliable and scalable NGS library preparation.