0
Thermo Scientific™ Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kits
Catalog No :
CAS Number :
Brand :
In Stock
Quantitation Range: 0.1 to 120 ng
Specifications:
| Application | Fluorescence-Based Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit provides an exceptionally accurate and sensitive method for quantifying low concentrations of double-stranded DNA (dsDNA). Designed specifically for fluorescence-based measurement using Qubit Fluorometers, this assay enables precise quantification of DNA samples that are too dilute for reliable measurement using traditional UV absorbance methods.
The kit features a ready-to-use 1X working solution, eliminating the need for reagent dilution and simplifying workflow. It offers outstanding selectivity for dsDNA over RNA, single-stranded DNA, proteins, and free nucleotides, ensuring accurate quantification even in the presence of contaminants commonly found in molecular biology samples.
With its ultra-sensitive detection range of 0.1–120 ng, the Qubit HS Assay Kit is ideal for next-generation sequencing (NGS), PCR, and other applications requiring accurate measurement of low-concentration DNA samples.
Key Features
Ultra-High Sensitivity for Low DNA Concentrations
- Detects dsDNA from as low as 0.1 ng
- Ideal for dilute or precious samples
- Ensures accurate quantification for critical sequencing workflows
Ready-to-Use 1X Assay Format
- Pre-formulated working solution eliminates dilution steps
- Reduces preparation time and improves workflow efficiency
- Ensures consistent and reproducible results
Exceptional Selectivity for Double-Stranded DNA
-
Highly selective for dsDNA over:
- RNA
- Single-stranded DNA
- Proteins
- Free nucleotides
- Minimizes interference from contaminants
Fast and Simple Workflow
- Requires only 1–20 μL sample volume
- Results available in minutes
- Minimal hands-on time
Superior Accuracy Compared to UV Absorbance Methods
- Fluorescence-based detection provides precise DNA quantification
- Ideal for sensitive downstream applications such as NGS library preparation
Applications
The Qubit 1X dsDNA HS Assay Kit is ideal for applications requiring precise quantification of low DNA concentrations, including:
- Next-generation sequencing (NGS) library preparation
- DNA quantification before Illumina sequencing
- PCR and qPCR workflows
- DNA extraction and purification workflows
- Genomic DNA quantification
- Molecular cloning applications
- Research in genomics, biotechnology, and molecular biology
Instrument Compatibility
Compatible with the following Invitrogen Qubit Fluorometers:
- Qubit™ 2 Fluorometer
- Qubit™ 3 Fluorometer
- Qubit™ 4 Fluorometer
- Qubit™ Flex Fluorometer
Kit Contents and Shipping
| Component | Shipping Condition | Storage |
|---|---|---|
| Qubit™ 1X dsDNA HS Working Solution | Ambient or controlled | Store as recommended |
| Qubit™ dsDNA HS DNA Standards | Wet ice | Refrigerate upon arrival |
Technical Specifications
| Parameter | Specification |
|---|---|
| Assay Type | dsDNA High Sensitivity (HS) |
| Quantitation Range | 0.1–120 ng |
| Detection Method | Fluorescence |
| Sample Volume | 1–20 μL |
| Number of Assays | 100 or 500 |
| Instrument Compatibility | Qubit 2, 3, 4, and Flex Fluorometers |
| Selectivity | Highly selective for dsDNA |
| Format | Ready-to-use 1X solution |
| Shipping Condition | Wet ice |
| Unit Size | Each |
Advantages for Genomics and Molecular Biology Laboratories
- Ultra-sensitive DNA quantification for low-concentration samples
- Essential for accurate Illumina NGS library preparation workflows
- Highly selective and reliable fluorescence-based detection
- Fast and easy workflow with minimal preparation
- Compatible with all Qubit Fluorometer models
- Ideal for sequencing, PCR, and molecular biology applications
The Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit is a powerful and reliable solution for precise quantification of low-concentration double-stranded DNA. Its high sensitivity, exceptional selectivity, and ready-to-use format make it an essential tool for genomics laboratories, sequencing facilities, and molecular biology researchers requiring accurate DNA measurement for critical applications such as next-generation sequencing, PCR, and cloning workflows.
- Pack Size: 500 assays 100 assays