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Invitrogen™ CD4 Monoclonal Antibody (RPA-T4), PE, eBioscience™
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ CD4 Monoclonal Antibody (Clone RPA-T4), PE-conjugated is a high-quality mouse IgG1κ antibody specific for human CD4, a 59 kDa cell surface receptor. CD4 is primarily expressed on T helper cells, thymocytes, and at low levels on monocytes, and serves as a receptor for HIV. The RPA-T4 clone blocks HIV binding and mixed lymphocyte reactions and recognizes a distinct epitope compared to OKT4, ensuring no cross-blocking.
This antibody is pre-titrated for flow cytometry, enabling consistent staining with minimal optimization. It excites at 488–561 nm and emits at 578 nm, making it compatible with blue, green, and yellow-green lasers.
Key Features
- Specific for human CD4 – detects CD4 on T helper cells, thymocytes, monocytes, and dendritic cells.
- Clone RPA-T4 – widely cited and validated in immunological research.
- PE-conjugated – provides strong, bright signal for flow cytometry applications.
- Distinct epitope recognition – different from OKT4; no cross-blocking.
- Pre-titrated format – tested for optimal performance at 5 µL/test (0.5 µg).
- Proven reliability – supported by 60+ published studies across immunology, oncology, and infectious disease research.
Applications and Tested Dilutions
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry | 5 µL/test (0.5 µg) |
(Optimal performance may require assay-specific titration.)
Technical Specifications
| Property | Value |
|---|---|
| Host / Isotype | Mouse / IgG1, κ |
| Clone | RPA-T4 |
| Conjugate | PE (Ex/Em 565/576 nm) |
| Concentration | 5 µL/test |
| Antibody Class | Monoclonal |
| Recommended Isotype Control | Mouse IgG1κ, PE (Clone P3.6.2.8.1) |
| Purification | Affinity chromatography |
| Form | Liquid |
| Storage Buffer | PBS, pH 7.2 with BSA and 0.09% sodium azide |
| Storage Conditions | 4 °C, protect from light, do not freeze |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| Reactivity | Human (published cross-use in mouse) |
| RRID | AB_1582249 |
| Catalog Numbers | 12-0049-42 (100 tests); 12-0049-41 (25 tests) |
Target Information
CD4 is a co-receptor for MHC class II molecules and a primary receptor for HIV. It plays a critical role in T-cell activation, immune response modulation, and pathogen recognition. CD4 expression is localized in the thymus, lymph nodes, spleen, tonsils, and also in non-lymphoid tissues such as the gut and brain. Multiple isoforms of CD4 have been described, with functions in immune defense and, in some contexts, neuronal injury in infectious and immune-mediated diseases.
- UniProt ID (Human): P01730
- Entrez Gene ID (Human): 920
Invitrogen™ CD4 Monoclonal Antibody (RPA-T4), PE, eBioscience™ is a reliable, highly cited tool for flow cytometry-based identification of CD4+ T cells and related populations. With its PE conjugation, validated performance, and epitope specificity, it is widely used in immunological studies, HIV research, and T-cell biology.
- Pack Size: For 100 Tests for 25 Tests