Invitrogen™ Dynabeads™ CD4 Positive Isolation Kit, 5mL

The Dynabeads™ CD4 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of high-purity CD4+ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells are released from the beads using the DETACHaBEAD™ reagent to obtain bead- and antibody-free cells. Advantages of the Dynabeads™ CD4 Positive Isolation Kit:

• Gentle isolation of CD4+ T cells from any sample—no columns required

• Bead- and antibody-free positive isolation of CD4+ cells for any downstream assay

About Dynabeads™ CD4 Positive Isolation Kit

The Dynabeads™ CD4 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 μm diameter) covalently coated with a monoclonal antibody specific for the CD4 membrane antigen predominantly expressed on the helper/ inducer subset of human T cells. The kit also includes a DETACHaBEAD™ reagent that releases the Dynabeads™ from the purified CD4+ cells. This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay.

Magnetic bead-based separation offers easy handling

Dynabeads™ are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet. The positively isolated cells are then washed, and DETACHaBEAD™ reagent is added to gently release the cells from the beads. This magnetic bead-based positive isolation method gives both bead- and antibody-free cells. The isolated CD4+ cells are phenotypically unaltered and suitable for any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD4+ cells. 

Specifications

• Cell Type: T Cells (CD4)
• Product Type: Positive Cell Isolation Kit
• Isolation Technology: Positive Isolation
• No. of Cells: Processes ∼2 x 109 cells total
• Output Viability: >95%
• Product Line: DETACHaBEAD™, DYNAL™, Dynabeads™
• Purity or Quality Grade: Research Grade
• Quantity: 5 mL
• Sample Type: PBMC, Blood
• Shipping Condition: Room Temperature
• Starting Material Cell No.: 1 x 107 PBMCs per isolation
• Target Species: Human
• Diameter (Metric): 4.5 μm
• Unit Size: 5 mL

Contents

• Each kit contains 5 mL Dynabeads™ CD4 coated with anti-CD4 monoclonal antibody, and 2 mL DETACHaBEAD™ CD4. The kit can process up to 2 x 109 cells.