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Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence)
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Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Plate Reader | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader is a versatile, modular instrument designed to meet the diverse analytical needs of bioscience and life science researchers.
Equipped with multiple detection technologies—Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen™, and Time-Resolved Fluorescence (TRF)—the Varioskan LUX provides unmatched performance, precision, and usability in microplate-based assays.
Engineered for flexibility and efficiency, the system supports spectral scanning, kinetic measurements, and multipoint readings while integrating automated features that simplify setup, optimize data accuracy, and enhance workflow reliability.
The instrument is HTRF™ certified (Cisbio Bioassays) and DLReady™ validated (Promega), ensuring compatibility with a wide range of assay formats for high-throughput and quantitative research applications.
Key Features and Benefits
- Five Measurement Technologies: Absorbance (UV-Vis), Fluorescence Intensity (including FRET), Luminescence, AlphaScreen/AlphaLISA, and Time-Resolved Fluorescence (TR-FRET/hTRF).
- Dual Optics System: Includes monochromator and filter-based optics for maximum flexibility and sensitivity.
- Spectral Scanning: Enables full-spectrum acquisition for assay optimization and peak wavelength identification.
- Multiple Reading Modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint readings for diverse applications.
- Smart Safety Controls: Automatic calibration, self-diagnostics, and safety features that prevent common experimental errors.
- Automatic Dynamic Range Selection: Adjusts signal sensitivity for each well, ensuring optimal detection accuracy.
- Integrated Gas Control (Optional): Regulates CO₂ and O₂ concentrations for cell-based assays.
- Real-Time Data Visualization: Displays spectra and kinetic curves during measurements for on-the-fly analysis.
- Validated System: Certified HTRF™ compatible and DLReady™ for enhanced assay reliability.
Software Features – SkanIt™ PC Software (Research Edition)
- License-Free Installation: Each user can install SkanIt™ on multiple workstations.
- Intuitive Interface: Simplifies workflow setup, data acquisition, and assay optimization.
- Virtual Pipetting Tool: Allows drag-and-drop plate layout configuration for easy experiment setup.
- Real-Time Data Monitoring: Continuous data storage prevents loss during power interruptions.
- Comprehensive Data Processing: Built-in calculations for blank subtraction, curve fitting, kinetics, and dose-response.
- Flexible Data Export: Supports export in .xlsx, .pdf, .xml, and .txt formats, or automatic email delivery post-run.
- Result Traceability: Full audit trail with traceable runtime actions and settings.
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Available Editions:
- Research Edition: For academic and life science laboratories.
- Drug Discovery Edition: Includes FDA 21 CFR Part 11 compliance features.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; TRF: Excitation 334 nm, Emission 400–700 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: >6 decades (top), >5.5 decades (bottom); TRF: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI (top): <0.4 fmol fluorescein/well; FI (bottom): <4 fmol fluorescein/well; TRF: <1 amol/well; Luminescence: <7 amol ATP/well |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well, 0–4 Abs at 450 nm), ±2% (384-well, 0–3 Abs) |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp |
| Shaking | Orbital |
| Temperature Control | Ambient +4°C to 45°C |
| Interface | PC Software (SkanIt Software) |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 57 kg (125 lb) |
| Plate Formats | 6–1536 well plates; µDrop plates |
| Accuracy (Photometric) | 0.003 Abs or ±2% (200–399 nm), ±1% (400–1000 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Power Requirements | 100–240 V, 50/60 Hz |
| Product Line | Varioskan™ |
| Unit Size | Each |
Applications
- Molecular Biology: DNA, RNA, and protein quantification (absorbance or fluorescence).
- Cell-Based Assays: Reporter gene analysis, cell viability, and cytotoxicity testing.
- Enzyme Kinetics: Kinetic assays for enzyme activity and inhibition studies.
- Drug Discovery and Screening: Quantitative high-throughput compound screening.
- Immunoassays: ELISA, AlphaLISA, and HTRF-based analysis.
- Luminescence Applications: ATP detection, gene expression, and bioluminescent cell assays.
Unique Advantages
- Modular and upgradeable—expand capabilities as your research grows.
- Rapid, reliable, and sensitive multimode measurements.
- Smart automation minimizes manual setup and operational errors.
- Comprehensive detection range for absorbance, fluorescence, luminescence, and TRF.
- Paired with SkanIt™ software for data integrity and flexibility.
- Certified compatibility ensures validated performance across assay platforms.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader is an advanced, modular, and upgradable microplate reader combining sensitivity, speed, and smart automation.
Supporting up to five detection technologies with dual optical systems, the Varioskan LUX enables comprehensive, high-accuracy analysis for molecular biology, biochemistry, and drug discovery applications.
Paired with SkanIt™ Software, it delivers intuitive control, powerful data analysis, and full traceability, setting a new standard in multimode microplate reading for modern research laboratories.