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Thermo Scientific™ Emission filters for Varioskan™ LUX Multimode Microplate Readers
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Specifications:
| Application | Spectrophotometry |
| Storage Temperature | Room Temperature |
| Product Type | Accessory |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Analytical grade |
Thermo Scientific™ Emission Filters are high-performance optical components designed specifically for the Varioskan™ LUX Multimode Microplate Readers. These filters enable precise wavelength selection for fluorescence and luminescence measurements, providing optimized performance for BRET, NanoBRET, HTRF, TR-FRET, AlphaScreen™, AlphaLisa™, and other multiplex assays.
Each filter is engineered for easy installation into the Varioskan LUX filter wheel, and their use is seamlessly integrated with SkanIt™ software for automated setup, error prevention, and streamlined workflows.
Key Features & Benefits
✅ Wide Range of Filter Options
- Covers fluorescence, luminescence, TRF, AlphaScreen/AlphaLisa, and multiplexing assays.
- Available for specific applications like luciferase detection, terbium or europium measurements, and BRET donor/acceptor detection.
✅ Application-Specific Precision
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Filters are optimized for common assay types:
- BRET / NanoBRET
- AlphaScreen / AlphaLisa
- HTRF / LanthaScreen™
- Luciferase reporter assays (e.g., Renilla, firefly, Gaussia, Cypridina)
✅ Effortless Installation & Configuration
- Easily integrated into the Varioskan LUX filter wheel.
- SkanIt software automates filter recognition and prevents setup errors.
✅ High Sensitivity & Selectivity
- Ensures maximum signal collection at target wavelengths.
- Essential for luminometric assays with multiple emission wavelengths.
✅ Robust & Versatile
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Compatible with any filter-based detection technology used in Varioskan LUX:
- Luminescence
- Time-resolved fluorescence (TRF)
- Alpha technologies
Available Emission Filters by Catalog Number
| Catalog No. | Wavelength | Application |
|---|---|---|
| F46080 | 460 nm | NanoBRET donor |
| F47040 | 470/40 nm | Cypridina / Gaussia luciferase assays |
| F48020 | 480 nm | BRET donor using wild-type Rluc |
| F49210 | 492 nm | Terbium measurement |
| F51060 | 510 nm | Chroma-Glo luciferase (CBG68, CBG99) |
| F51530 | 515 nm | BRET2 acceptor (GFP2) |
| F52010 | 520 nm | LanthaScreen or HTRF donor (TbGreen) |
| F52530 | 525 nm | Pierce green Renilla luciferase |
| F53030 | 530 nm | BRET acceptor (eYFP) |
| F57177 | 571 nm | AlphaScreen measurement |
| F61060 | 610 nm | Chroma-Glo luciferase (CBR assay) |
| F610LP | 610 nm | NanoBRET acceptor |
| F61520 | 615 nm | AlphaLisa measurement |
| F62010 | 620 nm | Europium (Eu) donor and acceptor, LanthaScreen, HTRF |
| F640LP | 640 nm | Pierce red firefly luciferase |
| F66510 | 665 nm | EuRed/TbRed HTRF or LanthaScreen acceptor |
Compatibility & Usage
| Instrument | Varioskan™ LUX Multimode Microplate Reader |
|---|---|
| Software Integration | SkanIt™ Software (automated filter setup) |
| Detection Technologies | Luminescence, TRF, AlphaScreen, TR-FRET |
| Filter Type | Emission filters (standard and longpass) |
| Filter Installation | Manual insertion; software-guided workflow |
Applications
- Bioluminescence Resonance Energy Transfer (BRET, NanoBRET)
- Time-Resolved Fluorescence (TRF, TR-FRET, HTRF)
- AlphaScreen™ and AlphaLisa™ Assays
- Luciferase Reporter Gene Assays
- Multiplex Assays with Dual or Triple Emission Detection
- LanthaScreen™ and Eu/Tb-based fluorometric detection
Why Choose Thermo Scientific™ Emission Filters for Varioskan™ LUX?
✅ Optimized wavelength detection for every assay type
✅ Streamlined installation and error-free setup with SkanIt™ software
✅ Wide selection of filters for flexible assay development
✅ Reliable performance with enhanced signal clarity
✅ Compatible with all filter-based detection modes in Varioskan LUX
These emission filters are essential accessories for researchers looking to expand the functional capabilities of their Varioskan™ LUX reader with assay-specific wavelength detection and multiplexing precision.
- Wavelenght: 492 nm for General Terbium measurement 520 nm for LanthaScreen measurement of Tb acceptor; HTRF donor measurement of TbGreen 620 nm for General Europium measurement; LanthaScreen measurement of Eu donor; HTRF acceptor measurement of TbGreen; HTRF donor measurement of EuRed and TbRed 665 nm for LanthaScreen measurement of Eu acceptor; HTRF acceptor measurement of EuRed and TbRed