0
Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence)
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | molecular biology, Spectrophotometry | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Plate Reader | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader is a powerful, modular, and upgradable platform designed for demanding life science research applications, offering up to five key measurement technologies including:
- Absorbance (UV-Vis)
- Fluorescence Intensity (incl. FRET)
- Luminescence
- AlphaScreen™/AlphaLISA™
- Time-Resolved Fluorescence (TRF, including TR-FRET/hTRF)
With automatic dynamic range selection, smart safety features, and SkanIt™ Software, the Varioskan LUX streamlines complex assay workflows, minimizes errors, and supports a wide range of experimental formats from 6 to 1536-well plates, including μDrop plates for micro-volume measurements. The system includes spectral scanning, endpoint, kinetic, and multipoint measurements, and is ideal for DNA/RNA/protein quantification, HTRF®, DLReady™, and cell-based assays.
Key Features & Benefits
✅ Versatile Detection Technologies
-
Combines up to 5 measurement modes:
- UV-Vis Absorbance (200–1000 nm)
- Fluorescence Intensity (200–840 nm)
- Time-Resolved Fluorescence (TR-FRET)
- Luminescence (360–670 nm)
- AlphaScreen™ / AlphaLISA™
✅ Top & Bottom Reading Options
- Adaptable to standard and low-volume plates, with optimized settings for each assay type.
✅ Fast & Precise Measurements
- Reads a 96-well plate in 15 seconds, 384 wells in 45 seconds, and 1536 wells in 135 seconds
-
High sensitivity:
- < 0.4 fmol fluorescein/well (fluorescence, top)
- < 7 amol ATP/well (luminescence)
- < 1 amol/well (TRF)
✅ Monochromator & Filter Flexibility
- Double monochromator for absorbance & fluorescence intensity
- Fixed filter excitation and emission for TRF
- Custom emission filters available for purchase
✅ Advanced Measurement Modes
- Endpoint, kinetic, spectral scans, kinetic spectra, multipoint
- Spectral scanning speed: <2.2 sec/well (400–500 nm, 1 flash, 2 nm steps)
✅ Integrated Smart Features
- Automatic dynamic range selection ensures optimal readings
- Smart safety controls warn users of potential errors
- Automated calibration and diagnostics
✅ SkanIt™ Software (License-Free)
- Compatible with all Thermo Scientific readers
- Easy data export in multiple formats (.xlsx, .pdf, .txt, .xml)
- Virtual Pipetting Tool and searchable protocol library
- Research & 21 CFR Part 11-compliant Drug Discovery Editions
Instrument Specifications
| Parameter | Value |
|---|---|
| Catalog Number | VLBL0TD0 |
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Dispensers | None |
| Reading Options | Top and Bottom |
| Plate Types Supported | 6–1536-well plates, μDrop Plates |
| Cuvette Compatibility | No |
| Incubation Range | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Light Source | Xenon Flash Lamp |
| Detector | Photomultiplier Tube (PMT) |
| Dynamic Range | Luminescence: >7 decades, TRF: >6 decades, Fluorescence: >6 decades |
| Wavelength Selection | Double monochromator (Abs, Fluor), Filters (TRF) |
| Wavelength Range | 200–1000 nm (Abs), 200–840 nm (Fluor), 334/400–700 nm (TRF), 360–670 nm (Lumi) |
| Photometric Accuracy | ±0.003 Abs or ±1–2% (depending on range) |
| Precision | <0.001 Abs SD or CV <0.5% |
| Linearity | ±2% (0–4 Abs @ 450 nm, 96-well plate) |
| Spectral Resolution | 1 nm |
| Spectral Bandwidth | <2.5 nm |
| Software | SkanIt Software (Research & Compliance Editions) |
| Cloud Integration | Yes (via SkanIt & Thermo Fisher Connect) |
| Dimensions (W×D×H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 57 kg (125 lbs) |
Applications
- Gene Expression & qPCR Assay Optimization
- DNA, RNA & Protein Quantification
- Enzyme Kinetics & Reaction Monitoring
- Cell Proliferation, Cytotoxicity & Viability Assays
- Reporter Gene Assays (Luciferase, β-gal)
- HTRF, AlphaScreen™, AlphaLISA™, TR-FRET Assays
- Multiplexed Biochemical Assays
- Drug Screening & Toxicology Studies
Certifications & Validation
- HTRF™ certified by Cisbio Bioassays
- DLReady™ validated by Promega
- 21 CFR Part 11 Compliance (Drug Discovery Edition)
Why Choose the Varioskan™ LUX?
✅ Combines multi-mode detection in one upgradeable system
✅ Delivers fast, sensitive, and reproducible results
✅ Designed with smart automation and user safety in mind
✅ Intuitive, license-free SkanIt™ Software simplifies setup & analysis
✅ Supports advanced assays with broad wavelength and format compatibility
The Varioskan LUX is ideal for academic, pharmaceutical, and biotech laboratories needing high-throughput, multi-assay compatibility with built-in intelligence for enhanced workflow confidence.