Sigma-Aldrich Penicillin G potassium salt, powder, suitable for cell culture, BioReagent

Sigma-Aldrich Penicillin G Potassium Salt, BioReagent – Suitable for Cell Culture

Broad-Spectrum β-Lactam Antibiotic for Microbiology & Cell Culture Applications

Sigma-Aldrich Penicillin G Potassium Salt (BioReagent, Cell Culture Grade) is a high-purity, broad-spectrum β-lactam antibiotic widely used in molecular biology, microbiology, and mammalian cell culture. It inhibits bacterial cell wall synthesis, making it an essential reagent for antibiotic selection, contamination prevention, and bacterial growth inhibition studies.

With potency ranging from 1440–1680 units per mg, this water-soluble antibiotic is ideal for cell culture supplementation, antimicrobial research, and pharmaceutical applications.

Key Features & Benefits

✅ High-Purity BioReagent Grade – Ensures consistent performance in cell culture & microbiology

✅ Broad-Spectrum β-Lactam Antibiotic – Effective against Gram-positive bacteria & some Gram-negative bacteria

✅ Cell Culture-Suitable – Prevents bacterial contamination in mammalian cell cultures

✅ Inhibits Cell Wall Synthesis – Binds to penicillin-binding proteins (PBPs), blocking peptidoglycan cross-linking

✅ Highly Soluble in Water (100 mg/mL) – Allows easy preparation of stock solutions

✅ Stable at -20°C for Extended Storage – Ensures long shelf life and activity retention

✅ Validated for Research & Drug Development – Supports bacterial selection, antimicrobial resistance studies, and pharmaceutical testing

Technical Specifications

Mode of Action & Resistance

Mechanism of Action:

• Penicillin G inhibits bacterial cell wall synthesis by binding to penicillin-binding proteins (PBPs).
• This prevents cross-linking of peptidoglycan chains, leading to cell lysis and bacterial death.
• Most effective against actively dividing bacterial cells.

Mode of Resistance:

• Bacterial β-lactamases hydrolyze Penicillin G, inactivating its function.
• Some bacteria alter PBPs, reducing antibiotic binding and conferring resistance.
• Resistance is common in penicillinase-producing Staphylococcus spp. and Enterococcus spp..

Antimicrobial Spectrum:

✔ Gram-Positive Bacteria (Streptococcus spp., Staphylococcus spp., Bacillus spp.)

✔ Gram-Negative Bacteria (Neisseria meningitidis, Haemophilus influenzae)

✔ Anaerobic Bacteria (Clostridium spp., Actinomyces spp.)

Applications

🔬 Microbiology & Bacterial Culture – Prevents contamination in bacterial culture media & microbiological assays

🦠 Mammalian Cell Culture Studies – Used in cell culture media to prevent bacterial contamination

⚗️ Antibiotic Resistance Research – Evaluates Penicillin G susceptibility in bacterial strains

🧪 Pharmaceutical & Drug Development – Used in antibiotic formulation & bacterial inhibition studies

🏭 Food & Beverage Safety Testing – Screens for penicillin-resistant bacterial contamination

Preparation & Storage Guidelines

1️⃣ Preparation of Penicillin G Stock Solution

• Dissolve 100 mg/mL in sterile water or PBS.
• Filter sterilize before use (do not autoclave).
• Store at 2–8°C (short-term) or -20°C (long-term) for extended stability.

2️⃣ Recommended Working Concentrations

• Bacterial Selection & Growth Inhibition: 10–100 µg/mL
• Mammalian Cell Culture Protection: 50–100 U/mL

3️⃣ Storage & Handling

• Keep tightly sealed in a dry, ventilated environment.
• Protect from moisture & high temperatures to prevent degradation.
• Avoid repeated freeze-thaw cycles for optimal stability.

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for clinical or therapeutic applications.

⚠️ Hygroscopic & Light-Sensitive – Store in a dry, well-ventilated area.

⚠️ Use Protective Equipment (PPE) – Avoid direct skin contact & inhalation.

Why Choose Sigma-Aldrich Penicillin G Potassium Salt (BioReagent, Cell Culture Grade)?

🔹 High-Purity Antibiotic (1440–1680 Units per mg) for Reliable Research

🔹 Effective Against Gram-Positive & Some Gram-Negative Bacteria

🔹 Validated for Mammalian Cell Culture & Bacterial Inhibition

🔹 Stable Under Proper Storage Conditions (-20°C for Extended Use)

🔹 Available in Multiple Pack Sizes for Research Flexibility

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀