Thermo Fisher Scientific Invitrogen™ Qubit™ 1X Dsdna Hs Assay Kit, 100 Assays Per Kit

The Invitrogen™ Qubit™ dsDNA Broad Range (BR) Assay Kits provide a highly accurate and selective fluorescence-based solution for quantifying double-stranded DNA (dsDNA) across a broad concentration range. Designed for use with Qubit Fluorometers, these kits enable reliable measurement of DNA concentration while minimizing interference from contaminants such as RNA, proteins, free nucleotides, and salts.

Unlike UV spectrophotometry, which measures total nucleic acid content and contaminants, the Qubit dsDNA BR assay uses DNA-selective fluorescent dyes that bind specifically to double-stranded DNA. This ensures precise quantification essential for sensitive downstream applications such as next-generation sequencing (NGS), PCR, cloning, and genomics research.

The assay is ideal for laboratories requiring accurate DNA quantification across a wide concentration range for molecular biology, biotechnology, and sequencing workflows.

Key Features

Broad Range DNA Quantification

• Quantifies dsDNA from 4 ng to 2,000 ng
• Suitable for moderate to high DNA concentration samples
• Ideal for routine molecular biology and genomics workflows

Highly Selective for Double-Stranded DNA

• Specifically detects dsDNA with minimal interference from:
  • RNA
  • Single-stranded DNA
  • Proteins
  • Free nucleotides
  • Contaminants

Superior Accuracy Compared to UV-Based Methods

• Fluorescence-based detection improves specificity
• Eliminates overestimation caused by contaminants
• Provides reliable results for critical downstream applications

Fast and Simple Workflow

• Requires only 1–20 μL sample volume
• Easy assay setup with ready-to-use reagents
• Rapid results using Qubit Fluorometer

Reliable Performance in Complex Samples

• Tolerates contaminants such as:
  • Salts
  • Solvents
  • Detergents
• Ensures consistent quantification results

Applications

The Qubit dsDNA BR Assay Kit is ideal for:

• DNA quantification for next-generation sequencing (NGS)
• Illumina library preparation workflows
• PCR and qPCR sample preparation
• DNA extraction and purification workflows
• Molecular cloning workflows
• Genomics and molecular biology research
• Biotechnology and pharmaceutical research

Instrument Compatibility

Compatible with all Qubit Fluorometers:

• Qubit™ 1.0 Fluorometer
• Qubit™ 2.0 Fluorometer
• Qubit™ 3 Fluorometer
• Qubit™ 4 Fluorometer
• Qubit™ Flex Fluorometer

Kit Contents and Storage

Provides sufficient reagents for 100 or 500 assays.

Technical Specifications

Advantages for Genomics and Sequencing Laboratories

• Highly accurate DNA quantification for NGS workflows
• Essential for Illumina sequencing library preparation
• Superior accuracy compared to UV spectrophotometry
• Fast and easy workflow
• Reliable performance across a broad concentration range
• Ideal for genomics, biotechnology, and research laboratories

The Invitrogen™ Qubit™ dsDNA Broad Range Assay Kits provide a fast, accurate, and reliable solution for quantifying double-stranded DNA across a wide concentration range. With fluorescence-based detection, high specificity, and compatibility with Qubit Fluorometers, these kits are essential tools for genomics, sequencing, and molecular biology laboratories requiring precise DNA quantification for critical research and analytical workflows.