Invitrogen™ Collibri™ ES DNA Library Prep Kit for Illumina™ Systems with UD Indexes

The Invitrogen™ Collibri™ ES DNA Library Prep Kit for Illumina Systems with UD (Unique Dual) Indexes is a complete and advanced solution for preparing high-quality, uniquely indexed DNA libraries for next-generation sequencing (NGS). Designed for maximum accuracy, flexibility, and throughput, this kit enables rapid and reliable library preparation from genomic DNA or amplicons (>300 bp) across a wide input range of 1–500 ng.

The inclusion of Unique Dual (UD) Indexes ensures superior multiplexing capability and significantly reduces index misassignment, also known as index hopping. This makes the kit especially suitable for high-throughput sequencing applications and sensitive workflows requiring maximum data integrity.

The streamlined enzymatic fragmentation (ES) workflow enables library preparation in approximately three hours using PCR amplification. PCR-free workflows are also supported for DNA inputs of ≥100 ng, allowing researchers to reduce bias and improve sequencing accuracy.

Key Features

Unique Dual Indexing for Maximum Accuracy

• Includes UD indexes to minimize index hopping and cross-contamination
• Ensures accurate sample identification in multiplex sequencing workflows
• Ideal for high-throughput sequencing and sensitive applications

Complete Library Preparation Kit

• Includes enzymatic fragmentation, cleanup reagents, and UD indexed adapters
• Fully integrated solution with all necessary components included
• Eliminates the need for separate indexing kits

Fast and Efficient Workflow

• Library preparation completed in approximately 3 hours
• Reduced hands-on time improves laboratory productivity
• Supports both PCR-based and PCR-free workflows

Wide DNA Input Compatibility

• Supports DNA inputs from 1 ng to 500 ng
• Compatible with genomic DNA and amplicons greater than 300 bp
• Suitable for samples from all species

Advanced Enzymatic Fragmentation Technology

• Controlled enzymatic shearing ensures consistent fragmentation
• Produces high-yield, high-quality sequencing libraries
• Enables reliable library quantification and multiple sequencing runs

Visual Workflow Monitoring

• Inert dyes provide color-based visual confirmation of workflow steps
• Improves accuracy and reduces preparation errors

Automation and High-Throughput Compatibility

• Compatible with automated liquid handling systems
• Supports miniaturized workflows to reduce reagent costs
• Ideal for core sequencing facilities and high-throughput laboratories

Applications

The Collibri ES DNA Library Prep Kit with UD Indexes is optimized for a wide range of genomics and sequencing applications, including:

• Whole genome sequencing (WGS)
• Genome resequencing
• De novo genome assembly
• Human genomics and precision medicine research
• Microbial and pathogen genome sequencing
• Population genomics
• Public health and epidemiological surveillance
• Academic and biotechnology research

Illumina Platform Compatibility

Fully compatible with Illumina next-generation sequencing systems, including:

• NovaSeq™ 6000
• NextSeq™ 550
• MiSeq™
• iSeq™
• HiSeq™ X, 2500, 3000, and 4000

Available Kit Configurations

Kit Contents and Storage

Ships on dry ice to preserve reagent integrity.

Technical Specifications

Advantages for Sequencing Laboratories

• Unique dual indexing improves sequencing accuracy and multiplexing reliability
• Fast, efficient workflow increases laboratory throughput
• High library yield ensures optimal sequencing performance
• Fully compatible with Illumina NovaSeq, NextSeq, MiSeq, and HiSeq platforms
• Automation-ready for high-throughput sequencing facilities
• Ideal for genomics, clinical research, public health, and biotechnology laboratories

The Invitrogen™ Collibri™ ES DNA Library Prep Kit for Illumina Systems with UD Indexes provides a comprehensive, reliable, and high-accuracy solution for preparing uniquely indexed DNA libraries for next-generation sequencing. With its advanced enzymatic fragmentation, integrated unique dual indexing, rapid workflow, and automation compatibility, this kit is an ideal choice for sequencing laboratories, research institutions, and genomics facilities seeking precise, scalable, and high-quality Illumina sequencing library preparation.