Thermo Scientific™ Pierce™ Protease and Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce™ Protease and Phosphatase Inhibitor Mini Tablets provide a comprehensive solution for protecting proteins during cell lysis, extraction, and purification workflows. Each tablet contains a carefully balanced combination of protease and phosphatase inhibitors, delivering broad-spectrum protection against both proteolytic degradation and dephosphorylation.

This formulation includes EDTA, enabling inhibition of metalloproteases in addition to other protease classes. Each mini tablet is designed for small-volume applications and is sufficient for 10 mL of lysis buffer or sample solution, making it ideal for precise and controlled experimental setups.

Key Features

Dual Protection System

• Simultaneous inhibition of proteases and phosphatases
• Prevents protein degradation and loss of phosphorylation
• Maintains structural and functional integrity of proteins

Broad-Spectrum Protease Inhibition

• Targets serine, cysteine, aspartic proteases, and metalloproteases
• Contains aprotinin, bestatin, E-64, leupeptin, and EDTA
• Ensures comprehensive protection during extraction

Effective Phosphatase Inhibition

• Inhibits serine/threonine and tyrosine phosphatases
• Includes sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and β-glycerophosphate
• Preserves phosphorylation-dependent signaling pathways

Convenient Mini Tablet Format

• Pre-measured tablets for accurate dosing
• Rapid dissolution into clear solution
• One tablet treats 10 mL of buffer or lysate

Optimized for Laboratory Workflows

• Compatible with Pierce BCA Protein Assay
• Suitable for detergent-based lysis buffers
• Integrates easily into standard protein extraction protocols

Applications

• Protein extraction and purification
• Cell lysis for signaling pathway analysis
• Phosphoproteomics and kinase studies
• Western blotting and immunoassays
• Enzyme activity and protein stability studies

Mechanism of Action

During cell lysis, endogenous proteases degrade proteins while phosphatases remove critical phosphate groups, affecting protein function and signaling pathways. These mini tablets combine inhibitors that target both enzyme groups:

Protease inhibitors:

• Aprotinin – serine proteases
• Bestatin – aminopeptidases
• E-64 – cysteine proteases
• Leupeptin – serine and cysteine proteases
• EDTA – metalloprotease inhibition

Phosphatase inhibitors:

• Sodium fluoride – serine/threonine phosphatases
• Sodium orthovanadate – tyrosine phosphatases
• Sodium pyrophosphate – broad phosphatase inhibition
• β-glycerophosphate – serine/threonine phosphatases

This dual-action system ensures maximum protection of proteins and their post-translational modifications.

Technical Specifications

Usage Instructions

• Add 1 tablet to 10 mL of lysis buffer or sample
• Allow to dissolve completely before use
• Use immediately for protein extraction or purification workflows

Product Information

Storage and Stability

• Store at 2°C to 8°C
• Stable for up to 12 months under recommended conditions
• Keep container tightly sealed to prevent moisture exposure

Related Products

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Thermo Scientific™ Pierce™ Protease and Phosphatase Inhibitor Mini Tablets provide a robust and convenient solution for comprehensive protein protection during laboratory workflows. With dual inhibition, EDTA-enhanced coverage, and a user-friendly mini tablet format, they are ideal for proteomics and signaling studies requiring high accuracy and reproducibility.