Thermo Scientific™ Pierce™ Protease Inhibitor Tablets, EDTA-free

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets, EDTA-free, are a convenient and effective solution for protecting proteins from degradation during cell lysis and protein extraction workflows. These tablets contain a broad-spectrum mixture of protease inhibitors that target multiple classes of proteolytic enzymes, ensuring preservation of protein integrity in both tissue and cultured cell samples.

Designed for ease of use and compatibility with downstream applications, each tablet dissolves rapidly to produce a clear solution and is sufficient for preparing 50 mL of lysis buffer or sample solution.

Key Features

Broad-Spectrum Protease Inhibition

• Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
• Protects proteins from enzymatic degradation during extraction
• Ensures integrity of target proteins for accurate downstream analysis

EDTA-Free Formulation

• Suitable for applications requiring metal-dependent enzymes or metalloproteins
• Avoids interference with metal-sensitive assays and workflows

Convenient and Ready-to-Use

• Pre-formulated tablets eliminate the need for complex reagent preparation
• Dissolves quickly to form a clear solution
• One tablet treats 50 mL of buffer or lysate

Compatible with Common Assays

• Fully compatible with Pierce BCA Protein Assay
• Suitable for use with detergent-based lysis buffers
• Can be combined with phosphatase inhibitors if required

Cost-Effective and Efficient

• Economical alternative to liquid inhibitor cocktails
• Long shelf life when stored at 2–8°C
• Consistent performance across batches

Applications

• Protein extraction from tissue samples
• Cell lysis in cultured cells
• Proteomics and protein analysis workflows
• Western blotting and immunoassays
• Enzyme activity studies and protein purification

Mechanism of Action

Proteases released during cell lysis can rapidly degrade proteins, compromising experimental results. Pierce Protease Inhibitor Tablets contain a combination of inhibitors that act on different protease classes:

• AEBSF – inhibits serine proteases
• Aprotinin – inhibits serine proteases
• Bestatin – inhibits aminopeptidases
• E-64 – inhibits cysteine proteases
• Leupeptin – inhibits serine and cysteine proteases
• Pepstatin A – inhibits aspartic proteases

This multi-target inhibition ensures comprehensive protection of proteins throughout the extraction process.

Technical Specifications

Usage Instructions

• Dissolve 1 tablet in 50 mL of lysis buffer or sample solution
• Mix until fully dissolved before use
• Add directly to cell lysates or extraction buffers prior to protein isolation

Product Information

Storage and Stability

• Store at 2°C to 8°C
• Stable for up to 12 months when properly stored
• Protect from moisture and contamination

Related Products

• Pierce Protease Inhibitor Tablets (standard formulation)
• Pierce Protease Inhibitor Mini Tablets, EDTA-free
• Pierce Phosphatase Inhibitor Mini Tablets
• Pierce Protease and Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets, EDTA-free, provide a reliable and user-friendly solution for protecting proteins during extraction and analysis. With broad-spectrum inhibition, excellent compatibility, and convenient tablet format, they are an essential component for laboratories performing protein research and proteomics workflows.