Thermo Scientific™ Pierce™ Protease Inhibitor Tablets

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets are a convenient, ready-to-use solution designed to protect proteins from proteolytic degradation during cell lysis and protein extraction. These tablets contain a broad-spectrum formulation of protease inhibitors that effectively inhibit multiple classes of proteases commonly released during sample preparation.

Optimized for laboratory efficiency, each tablet dissolves rapidly to form a clear solution and is sufficient for preparing 50 mL of lysis buffer or sample solution, ensuring reliable and reproducible protein preservation across a wide range of applications.

Key Features

Broad-Spectrum Protease Protection

• Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
• Prevents degradation of target proteins during extraction
• Maintains protein integrity for downstream analysis

Convenient Tablet Format

• Pre-measured tablets eliminate preparation errors
• Rapid dissolution into clear solution
• One tablet sufficient for 50 mL of buffer or lysate

Optimized for Laboratory Workflows

• Compatible with detergent-based lysis buffers
• Suitable for use with Pierce BCA Protein Assay
• Easily integrated into standard protein extraction protocols

Cost-Effective and Reliable

• Economical alternative to liquid inhibitor cocktails
• Consistent batch-to-batch performance
• Long shelf life under recommended storage conditions

Applications

• Protein extraction from tissues and cultured cells
• Cell lysis workflows
• Proteomics and protein characterization studies
• Western blotting and immunoassays
• Enzyme and protein stability studies

Mechanism of Action

During cell lysis, proteases are released and can rapidly degrade proteins of interest. Pierce Protease Inhibitor Tablets contain a combination of inhibitors that target different protease classes:

• AEBSF – serine protease inhibitor
• Aprotinin – serine protease inhibitor
• Bestatin – aminopeptidase inhibitor
• E-64 – cysteine protease inhibitor
• Leupeptin – serine and cysteine protease inhibitor
• Pepstatin A – aspartic protease inhibitor

This multi-target inhibition ensures comprehensive protection of proteins during sample preparation.

Technical Specifications

Usage Instructions

• Dissolve 1 tablet in 50 mL of buffer or lysate
• Mix until fully dissolved before use
• Add to lysis buffer prior to protein extraction

Product Information

Storage and Stability

• Store at 2°C to 8°C
• Stable for up to 12 months under recommended conditions
• Keep tightly sealed to prevent moisture exposure

Related Products

• Thermo Scientific Pierce Protease Inhibitor Tablets EDTA-free
• Pierce Protease Inhibitor Mini Tablets
• Pierce Phosphatase Inhibitor Mini Tablets
• Pierce Protease and Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets provide a reliable, easy-to-use solution for protecting proteins during extraction and analysis. With broad-spectrum inhibition, excellent compatibility, and a convenient tablet format, they are an essential reagent for laboratories engaged in protein research and proteomics workflows.