Thermo Scientific™ Pierce™ Alkaline Phosphatase, Biotinylated 1mg

Thermo Scientific™ Pierce™ Biotinylated Alkaline Phosphatase (B-AP) is a high-purity, enzymatically active biotin-conjugated enzyme designed for sensitive detection in immunohistochemical (IHC) and biochemical assays. The biotin label enables specific binding to avidin, streptavidin, or NeutrAvidin™-based detection systems, making it an essential reagent in avidin-biotin complex (ABC) methods.

This biotinylated form of alkaline phosphatase (AP) is most commonly used for signal amplification in IHC, ELISA, Western blotting, and other applications where enzyme-linked detection is required. It offers an alternative to horseradish peroxidase (HRP), particularly for chromogenic substrates such as BCIP/NBT that yield intense, long-lasting colorimetric results.

Key Features & Benefits

• Biotinylated enzyme for ABC systems: Designed for high-affinity binding to avidin/streptavidin for enhanced signal generation.
• Optimized for IHC and ELISA: Amplifies signal when paired with biotinylated antibodies in standard ABC protocols.
• High Enzymatic Activity: Delivers robust performance with compatible substrates such as BCIP/NBT or pNPP.
• Convenient Format: Supplied as 1 mg of lyophilized powder for long shelf life and flexibility in dilution.
• Validated for Multiplexing: Ideal for dual-label experiments in combination with other enzyme-conjugated reporters.

Applications

• Immunohistochemistry (IHC) – Detection and amplification of biotinylated primary antibodies in tissue sections.
• ELISA – Enzymatic detection in sandwich or competitive formats using biotin-avidin interactions.
• Western Blotting – Visual detection of target proteins using chromogenic substrates.
• In Situ Hybridization (ISH) – Enzyme-linked detection of labeled DNA or RNA probes.

Storage & Stability

• Storage: Store at or below –20°C in a desiccated environment.
• Form: Lyophilized solid.
• Shelf Life: Stable for long-term storage under recommended conditions.

Thermo Scientific™ Pierce™ Biotinylated Alkaline Phosphatase is an essential detection reagent for researchers using avidin-biotin systems. With its high activity, biotin conjugation, and compatibility with a variety of chromogenic substrates, it enables sensitive and specific detection of biomolecules in a wide range of immunoassay applications.