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Thermo Scientific™ Pierce™ Bovine Serum Albumin, Biotinylated, 25 mg
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Specifications:
| Application | Protein Biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Recombinant Protein | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Pierce™ Bovine Serum Albumin, Biotinylated (B-BSA) is a highly purified protein conjugated with biotin, designed for use as a positive control or blocking agent in biotin-avidin detection systems. The biotinylation of BSA provides a consistent and stable reagent ideal for applications in immunohistochemistry (IHC), ELISA, Western blotting, and fluorescent assays involving streptavidin or avidin conjugates.
Each molecule of BSA is conjugated with multiple biotin moieties, allowing for strong binding with avidin/streptavidin and thereby enabling signal amplification or assay validation in biochemical and cell-based experiments.
Key Features & Benefits
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Pre-Conjugated Protein Control
– Biotinylated BSA serves as a ready-to-use positive control for biotin quantification and binding assays. -
High Affinity & Compatibility
– Binds with high specificity to streptavidin, avidin, and NeutrAvidin™ for avidin-biotin complex (ABC)-based detection systems. -
Stable and Reproducible
– Lot-to-lot consistency ensures reliable experimental outcomes in quantitative or comparative studies. -
Versatile Application Use
– Suitable for use in ELISA blocking buffers, antibody labeling controls, and validation of biotinylation workflows.
Applications
- Positive control in biotin quantitation assays
- Blocking agent in avidin/streptavidin-based immunoassays
- Standard reference in ABC-enhanced detection methods
- Component in labeling systems for protein–protein interaction studies
Product Specifications
| Property | Description |
|---|---|
| Label | Biotin |
| Protein | Bovine Serum Albumin (BSA) |
| Molecular Weight | ~66 kDa (BSA base) |
| Form | Lyophilized solid |
| Conjugation Ratio | Multiple biotin groups per BSA molecule |
| Application | IHC, ELISA, Western blot, biotin control assays |
| Storage Conditions | 2–8°C (refrigerator) |
Thermo Scientific™ Pierce™ Bovine Serum Albumin, Biotinylated provides a high-quality, biotin-labeled protein ideal for enhancing the accuracy and reproducibility of biotin-based detection systems. Whether used in assay development, system validation, or as a blocking reagent, B-BSA is a trusted control that supports reliable performance across a wide range of life science research applications.