0
Specifications:
| Application | Nucleic Acid Labeling and Detection | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₃₃H₃₂N₄O₈S |
Invitrogen™ Biotin-4-Fluorescein is a fluorescent biotin derivative designed for sensitive and quantitative assessment of biotin-binding proteins, such as avidin and streptavidin. Upon binding to avidin or streptavidin, the fluorescein signal is significantly quenched, allowing for straightforward and reproducible quantification of available binding sites.
This reagent is particularly useful in:
- Avidin-biotin titration assays
- Quantification of biotinylated surfaces or molecules
- Fluorescence-based affinity binding studies
The fluorescein tag enables use in applications involving fluorescence detection, microscopy, spectroscopy, or plate-based fluorescence assays.
Key Features & Benefits
- Specificity: High-affinity binding to avidin or streptavidin
- Quantitative: Allows precise measurement of biotin-binding site availability through fluorescence quenching
- Versatility: Applicable in both solution-based and surface-bound assays
- Spectrally Matched to FITC: Compatible with standard FITC filter sets for excitation/emission (494/523 nm)
- Convenient Storage: Stable at room temperature when protected from light and moisture
Recommended Use
To prepare a concentrated 1 mM stock solution, dissolve the lyophilized powder in anhydrous DMSO, DMF, or 100% methanol. Store the solution frozen, desiccated, and protected from light to maintain reagent integrity.
Specifications
| Property | Value |
|---|---|
| Product Name | Biotin-4-Fluorescein |
| Catalog Number | B10570 |
| Quantity | 5 mg |
| Molecular Weight | 644.7 g/mol |
| Excitation (Ex) | 494 nm |
| Emission (Em) | 523 nm |
| Fluorescent Label | FITC (Fluorescein) |
| Solubility | DMSO, DMF, or 100% methanol |
| Storage Conditions | Room temperature, protect from light |
| Shipping Condition | Ambient (Room temperature) |
| Intended Use | For Research Use Only |
Applications
- Quantifying avidin/streptavidin binding capacity
- Biotin-binding affinity studies
- Surface biotin density determination
- FITC-compatible fluorescence assays
- Bioanalytical method development
Invitrogen™ Biotin-4-Fluorescein offers a highly specific and reliable tool for quantitative biotin-binding studies using a fluorescence-based readout. Its compatibility with FITC filter sets and the simplicity of quenching-based detection make it ideal for researchers conducting binding kinetics, assay validation, or reagent quality control in a wide range of molecular biology and biochemistry applications.