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Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO
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Specifications:
| Application | Protein Purification |
| Storage Temperature | Room Temperature |
| Product Type | Dialysis Products |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes (3.5K MWCO) provide an efficient and convenient solution for removing salts and small contaminants from proteins and other macromolecules larger than 3,500 Daltons. Designed as an easy-to-use alternative to traditional dialysis tubing, these cassettes enable fast buffer exchange, desalting, equilibrium dialysis, and sample concentration with minimal sample loss.
The unique flat cassette chamber and dual-membrane design provide a high surface area-to-volume ratio for rapid dialysis, while the rectangular frame ensures maximum sample recovery from any of the four corner injection ports. Color-coded pink frames make identification of the 3.5K MWCO membrane quick and easy.
Key Features & Benefits
- Simple Setup – No knots, clips, or clamps needed; load samples through the self-sealing gasket with a syringe.
- Fast Dialysis – Flat chamber with dual membranes accelerates diffusion compared to cylindrical tubing.
- High Recovery Rate – Rectangular cassette shape ensures nearly complete sample retrieval.
- Flexible Volume Options – Available in 0.5 mL, 3 mL, 12 mL, and 30 mL capacities.
- Chemical Compatibility – Membrane and frame materials compatible with most laboratory buffers and reagents.
- Color-Coded Identification – Pink cassette frame denotes 3.5K MWCO.
Membrane Specifications
| Property | Specification |
|---|---|
| Composition | Regenerated cellulose (viscose method) |
| Thickness | 0.9–1.2 mil (22.5–30 μm) |
| Hydration Time | 2 minutes before use |
| Glycerol Content | None |
| Sulfur Content | 0.1–0.15% |
| Heavy Metals | Trace |
Available Models
| Catalog Number | Sample Volume Range | Nominal Capacity | Quantity per Pack |
|---|---|---|---|
| 66333 | 0.1–0.5 mL | 0.5 mL | 10 cassettes |
| 66330 | 0.5–3 mL | 3 mL | 10 cassettes |
| 66110 | 3–12 mL | 12 mL | 8 cassettes |
| 66130 | 12–30 mL | 30 mL | 6 cassettes |
Applications
- Buffer exchange for proteins and macromolecules
- Removal of salts and low-molecular-weight contaminants
- Protein desalting and sample concentration
- Equilibrium dialysis in biochemical research
Product Specifications
| Parameter | Detail |
|---|---|
| Product Line | Slide-A-Lyzer™ |
| Membrane Type | Cellulose, Dialysis |
| MWCO | 3.5 kDa |
| Format | Cassette |
| Purification Target | Buffer exchange, protein purification |
| Unit Size | Each |
| Storage Conditions | Room temperature |
| Intended Use | For research use only. Not for use in diagnostic procedures. |
Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, deliver high-efficiency removal of small molecules from proteins and macromolecules larger than 3,500 Da. Their easy-to-use design, fast dialysis capability, and high sample recovery make them an excellent choice for protein desalting, buffer exchange, and concentration workflows in research laboratories.
- Capacity: 0.5 mL, 10 Cassettes 3 mL, 10 Cassettes 12 mL, 8 Cassettes 30 mL, 6 Cassettes