Thermo Scientific™ Pierce™ BCA Protein Assay Kit

The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay designed to determine protein concentration in solutions. It uses the Bicinchoninic Acid (BCA) method, which combines the reduction of Cu²⁺ to Cu¹⁺ by proteins in an alkaline medium, followed by colorimetric detection of the reduced copper ion. The Pierce BCA Assay provides accurate and reliable protein quantification, with a linear range from 20 to 2000 μg/mL of BSA (Bovine Serum Albumin).

This kit is ideal for protein quantitation in biological and chemical applications, as it is highly sensitive, enabling detection down to 5 μg/mL. The colorimetric assay works well with most ionic and non-ionic detergents, making it suitable for a wide variety of samples.

Key Features:

1. Colorimetric Detection:
   • The BCA method results in a purple-colored complex that exhibits strong absorbance at 562 nm, providing precise protein quantification.
2. Fast and Simple Protocol:
   • The assay is quick, with a short incubation time compared to traditional methods (e.g., Lowry), providing fast results.
3. Detergent-Compatible:
   • The BCA assay is compatible with most detergents, including ionic and non-ionic detergents, making it ideal for protein samples prepared in buffer solutions containing detergents.
4. High Sensitivity:
   • The assay can detect protein concentrations as low as 5 μg/mL, offering high sensitivity for low-concentration samples.
5. Flexible for Different Applications:
   • The kit includes pre-diluted BSA standards and is available in several formats, allowing for use in microplate assays or tube assays, depending on the user's needs.
6. Quantitative Results:
   • The kit provides a quantitative measurement of protein concentration in solution, which is essential for protein quantification, purity determination, and protein yield assessments in laboratory studies.

Specifications:

Applications:

1. Protein Quantification:
   • Ideal for measuring protein concentration in biological, chemical, and industrial applications.
2. Cellular and Biochemical Research:
   • Useful for assessing protein yields, enzyme activity, and purity in biochemical assays.
3. Quality Control:
   • Employed in pharmaceutical and food industries to ensure protein quality and consistency.
4. Research Laboratories:
   • Used in academic research, proteomics, and biotechnology to analyze and quantify proteins in a variety of experimental conditions.
5. Metabolic Studies:
   • Useful for studying metabolic processes and protein dynamics in cellular and tissue samples.

Ordering information;

The Pierce BCA Protein Assay Kit is a highly reliable and versatile tool for protein quantification. With its detergent compatibility, high sensitivity, and simple protocol, it is perfect for use in laboratories, industrial applications, and research environments that require accurate and rapid protein concentration determination. The kit’s flexibility and wide compatibility with different sample types make it an essential reagent for protein analysis.