Liofilchem Egg Yolk Emulsion

The Liofilchem™ Egg Yolk Emulsion is a sterile, stabilized 50% egg yolk emulsion specifically designed as a liquid supplement for detecting microbial lecithinase activity. It is widely used in microbiological media formulations to support the identification of lecithinase-producing bacteria such as Bacillus cereus and Clostridium perfringens. This product conforms to international standards, including ISO 7932, ISO 21871, and FDA-BAM, ensuring accuracy and reliability in microbiological analysis.

Key Features

1. Sterile and Stabilized:
   • Ready-to-use emulsion ensures sterility and stability, enhancing reproducibility in test results.
2. Supports Lecithinase Detection:
   • Enables identification of lecithinase-positive organisms, such as Bacillus cereus and Clostridium perfringens, by detecting the hydrolysis of lecithin in the medium.
3. Versatile Applications:
   • Compatible with multiple culture media, including:
     • PEMBA (Polymyxin Pyruvate Egg Yolk Mannitol Bromothymol Blue Agar).
     • MYP (Mannitol Egg Yolk Polymyxin Agar).
     • TSC Agar (Tryptose Sulfite Cycloserine Agar).
     • SFP Agar (Shahidi-Ferguson Perfringens Agar).
4. ISO-Compliant:
   • Conforms to ISO 7932 and ISO 21871 for Bacillus cereus detection and enumeration.
5. Easy to Use:
   • Simply add to sterilized medium cooled to 45-50°C, mix thoroughly, and pour into Petri dishes.

Typical Composition (Per Litre)

Applications

1. Food Microbiology:
   • Detection of lecithinase-producing bacteria in food samples, including dairy and processed products.
2. Clinical Microbiology:
   • Identification of Clostridium perfringens and Bacillus cereus in clinical specimens.
3. Environmental Testing:
   • Monitoring microbial contamination in water and food production environments.
4. Pharmaceutical Microbiology:
   • Quality control testing for microbial contaminants in pharmaceutical products.

Preparation Instructions

1. Shake the bottle well to resuspend any sediment.
2. Aseptically add the required volume of Egg Yolk Emulsion to sterilized and cooled (45-50°C) culture medium.
3. Mix thoroughly and pour into sterile Petri dishes.

Compatible Media

• PEMBA: For detecting Bacillus cereus according to ISO 7932 and ISO 21871.
• MYP Agar: For identifying lecithinase-positive Bacillus cereus.
• TSC Agar: For detecting Clostridium perfringens in food and water samples.
• SFP Agar: For Clostridium perfringens enumeration in environmental samples.

Storage and Shelf Life

• Storage: Store at 2-8°C, protected from light.
• Shelf Life: 1 year.

Quality Control

• Appearance:
  • Yellowish opaque emulsion. Precipitate, if present, can be resuspended by shaking.
• Performance:
  • Validated using control strains:
    • Bacillus cereus (WDCM 00001/ATCC® 11778).
    • Escherichia coli (WDCM 00012/ATCC® 8739).
    • Bacillus subtilis (WDCM 00003/ATCC® 6633).

Advantages

• Reliable Performance: Ensures consistent detection of lecithinase activity.
• Versatile Compatibility: Works with a wide range of selective media.
• Ready-to-Use: Eliminates preparation steps, saving time and effort.
• ISO-Compliant: Meets international standards for food safety and microbiology.

Packaging

Warnings and Precautions

1. For In Vitro Diagnostic Use (IVD).
2. Use only by trained professionals.
3. Do not use if the product shows signs of contamination or deterioration.
4. Consult the Safety Data Sheet (SDS) for safe handling and disposal practices.

Why Choose Liofilchem Egg Yolk Emulsion?

• Trusted for detecting lecithinase-positive bacteria.
• Conforms to ISO and FDA-BAM standards for food safety.
• Backed by Liofilchem’s expertise in high-quality microbiological products.