Invitrogen™ CSPD™ Substrate (25 mM Concentrate)

Highly sensitive chemiluminescent substrate for alkaline phosphatase detection in blotting and solution-based assays

The Invitrogen™ CSPD™ Substrate (25 mM Concentrate) is a high-performance chemiluminescent substrate for alkaline phosphatase (AP) detection, offering exceptional sensitivity, low background, and long-lasting signal. It is ideal for protein and nucleic acid blotting on nitrocellulose membranes, including Southern, Northern, and Western blotting, as well as solution-based assays such as immunoassays, DNA probe assays, enzyme assays, and reporter gene assays.

Compared to traditional substrates such as methylumbelliferyl phosphate (MUP) and p-nitrophenyl phosphate (pNPP), CSPD™ delivers higher sensitivity and superior signal-to-noise performance. Maximum light output is achieved within approximately 10 minutes, with glow emission persisting for several hours. For optimal performance in solution assays and certain membrane applications, use with chemiluminescent enhancers is recommended.

Key Features & Benefits

• Ultra-Sensitive Detection – Detects alkaline phosphatase with unparalleled sensitivity in both blotting and solution-based formats.
• Low Background, High Signal – Maximizes signal-to-noise ratio for clear and reliable results.
• Fast Kinetics – Achieves maximum light output in ~10 minutes.
• Long-Lasting Glow – Stable luminescence for several hours, enabling flexible imaging times.
• Versatile Application – Suitable for Southern, Northern, and Western blots, as well as immunoassays and reporter gene assays.
• Improved Over Conventional Substrates – Outperforms MUP and pNPP in sensitivity and ease of use.

Applications

• Southern Blotting – Detection of specific DNA sequences.
• Northern Blotting – Detection of specific RNA sequences.
• Western Blotting – Protein detection using AP-conjugated antibodies.
• Solution-Based Assays – Immunoassays, DNA probe assays, enzyme assays, reporter gene assays.

Technical Specifications

Ordering Information

The Invitrogen™ CSPD™ Substrate (25 mM Concentrate) provides a fast, sensitive, and versatile solution for detecting alkaline phosphatase in both membrane-based and solution-based assays. Its high light intensity, long glow duration, and low background make it an excellent choice for researchers requiring consistent, high-quality results across a range of molecular biology applications.