Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X) is a high-performance electrophoresis buffer specifically formulated for use with NuPAGE™ Bis-Tris gels. This buffer is optimized for separating small- to medium-sized proteins under denaturing SDS-PAGE conditions, offering faster protein migration than traditional MOPS-based buffers.

The MES (2-(N-morpholino)ethanesulfonic acid) system maintains a near-neutral pH (7.3) during electrophoresis, improving protein stability while enabling sharp band resolution. When diluted to 1X, the buffer provides consistent and reproducible results for downstream Western blotting, protein analysis, or mass spectrometry.

Key Features and Benefits

• Optimized for NuPAGE™ Bis-Tris gels
• Fast migration of small to mid-sized proteins
• Improved resolution due to effective stacking and separation
• Pre-made 20X concentrate – easy dilution to 1X working solution
• pH 7.3 stability – preserves protein structure during electrophoresis
• Compatible with MES-compatible markers and precast gel systems

Applications

• SDS-PAGE protein separation
• Western blotting sample prep
• High-resolution analysis of small proteins
• Ideal for workflows involving NuPAGE™ precast gels

Buffer Comparison Note

• MES SDS buffer provides faster migration for small proteins
• MOPS SDS buffer is better suited for larger proteins
• Choose MES for applications prioritizing sharp resolution of low-molecular weight proteins

Technical Specifications

NuPAGE™ MES SDS Running Buffer (20X) is the ideal choice for fast and sharp separation of small to mid-sized proteins using NuPAGE™ Bis-Tris gels. Its reliable formulation and ease of use make it a trusted buffer for routine protein electrophoresis and downstream analysis.