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Specifications:
| Application | Protein Electrophoresis & Western Blotting |
| Storage Temperature | Room Temperature |
| Product Type | Accessory |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The Invitrogen™ XCell II™ Blot Module is a mini-gel wet transfer unit designed for use with the XCell SureLock™ Mini-Cell or XCell II Gel Tank systems. It provides a simple, efficient, and consistent method for transferring proteins or nucleic acids from polyacrylamide gels to membranes in western, southern, or northern blotting workflows.
With a maximum blot size of 9 × 9 cm and requiring less than 200 mL of transfer buffer, the XCell II Blot Module offers a cost-effective solution for standard blotting applications. The system features rugged platinized titanium and stainless-steel electrodes that ensure a uniform electric field for reliable and reproducible transfers—without clamps or hinged gel holders.
Key Features & Benefits
✅ Compact Wet Transfer System
- Fits easily into XCell SureLock™ Mini or XCell II Mini Gel Tanks.
- Allows wet transfer of up to 2 mini gels (8 × 8 cm) simultaneously.
✅ Low Buffer Volume Requirement
- Requires <200 mL of transfer buffer, reducing reagent consumption and cost.
✅ Efficient & Uniform Transfer Performance
- Platinized titanium anode and stainless-steel cathode electrodes deliver even current distribution for consistent transfer quality.
- Ensures sharp band resolution and high transfer efficiency for both proteins and nucleic acids.
✅ Clamp-Free, User-Friendly Design
- No need for clamps or hinged holders; fast setup and takedown.
- Minimizes potential for transfer errors and ensures leak-free operation.
✅ Versatile Gel & Membrane Compatibility
- Compatible with Bolt™, NuPAGE™, and Novex™ mini gels.
- Works with PVDF and nitrocellulose membranes for protein blotting.
Product Specifications
| Feature | Details |
|---|---|
| Product Name | XCell II™ Blot Module |
| Catalog Number | EI9051 |
| Transfer Type | Wet Transfer |
| Compatible Equipment | XCell SureLock™ Mini-Cell, XCell II Tank |
| Max. Blot Size | 9 × 9 cm |
| Gel Size | Mini (8 × 8 cm) |
| Buffer Volume Required | <200 mL |
| Electrodes | Platinized titanium (anode), stainless steel (cathode) |
| Capacity | Up to 2 mini gels per run |
| Transfer Mode | Vertical |
| Gel Compatibility | Bolt™, NuPAGE™, Novex™ Gels |
| Shipping Conditions | Room Temperature |
| Unit Size | Each |
| Product Line | XCell II™ |
Kit Contents
- Anode core
- Cathode core
- 8 Sponge pads
- Instruction manual
Note: Requires XCell SureLock Mini-Cell or XCell II Gel Tank to operate (not included).
Applications
- Western blotting: Transfer of proteins from SDS-PAGE gels to membranes.
- Southern blotting: DNA transfer for hybridization and detection.
- Northern blotting: RNA transfer and analysis.
- Routine molecular biology and proteomics workflows.
How It Works
- Insert gel and membrane into transfer sandwich with sponge pads.
- Place the sandwich between the anode and cathode cores.
- Submerge the assembled module into the mini gel tank filled with buffer.
- Apply power using the electrophoresis power supply to begin transfer.
- Monitor transfer time based on protocol (typically 1–2 hours).
Why Choose the XCell II™ Blot Module?
✅ Efficient wet transfer system compatible with mini gels
✅ Low buffer volume reduces reagent usage and cost
✅ Even, reliable transfer with precision-engineered electrodes
✅ Clamp-free design for faster, user-friendly setup
✅ Ideal for routine western, southern, or northern blotting workflows
The Invitrogen™ XCell II™ Blot Module is the perfect addition for labs looking for high-quality, reliable protein or nucleic acid transfers using a compact, affordable wet transfer system.