Thermo Scientific™ Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits

Thermo Scientific™ Hanks' Balanced Salt Solution (HBSS) without Ca²⁺, Mg²⁺ is an essential buffer solution used in primary cell isolation, cell washing, and tissue dissociation. This formulation is specifically optimized for Pierce™ Primary Cardiomyocyte Isolation Kits, providing high cell viability and yield during enzymatic tissue digestion and culture preparation.

HBSS without calcium and magnesium prevents premature cell attachment and is ideal for gentle washing of primary cells after connective tissue digestion, ensuring optimal recovery of viable cells for downstream applications such as cardiomyocyte differentiation, immunohistochemistry (IHC), and functional assays.

Key Features & Benefits

1. Optimized for Primary Cardiomyocyte Isolation & Culture
   • Supports efficient digestion and dissociation of cardiac tissue.
   • Prevents premature cell adhesion during isolation procedures.
2. Gentle, Balanced Buffer for Primary Cell Washing
   • Maintains osmotic balance and physiological pH during cell processing.
   • Minimizes cell stress and damage during enzymatic digestion steps.
3. Improves Cell Viability & Yield
   • Provides 1.5- to 4-fold higher cell yield compared to DIY isolation methods.
   • Ensures high viability and functional activity of isolated cardiomyocytes.
4. Time-Saving & Efficient
   • Reduces processing time to under 2 hours, compared to >16 hours with traditional protocols.
   • Works in conjunction with Pierce™ Cardiomyocyte Isolation Enzymes and Growth Supplements.
5. Sterile & Ready-to-Use
   • Provided as a pre-filtered, sterile liquid solution, eliminating the need for additional preparation.
   • Stored at 4°C to ensure long-term stability.

Product Specifications

Use Cases & Applications

1. Primary Cell Isolation & Cardiomyocyte Culture

• Enhances Yield & Viability of Isolated Cardiomyocytes
  • Prevents cell attachment during digestion for improved isolation efficiency.
  • Used in neonatal mouse and rat cardiomyocyte isolation protocols.
• Facilitates Enzymatic Tissue Dissociation
  • Supports protease-based dissociation steps (e.g., papain, thermolysin, collagenase treatments).
  • Works in conjunction with Cardiomyocyte Isolation Enzyme 1 & 2.

2. Immunohistochemistry (IHC) & Functional Assays

• Prepares Primary Cells for Immunohistochemical Staining
  • Provides a stable, physiologically buffered environment for sample processing.
  • Reduces nonspecific cell lysis during antibody labeling procedures.
• Maintains Cell Integrity for Functional Assays
  • Ensures optimal conditions for preclinical drug discovery and disease modeling.
  • Supports in vitro cardiac electrophysiology and contraction assays.

3. Tissue Processing & Preclinical Drug Discovery

• Biologically Relevant System for Cardiovascular Research
  • Preserves functional activity of cardiomyocytes for contraction studies.
  • Enables toxicology and ischemia-related research in vitro.
• Enhances Primary Cell-Based Screening Assays
  • Works in high-throughput screening models using cardiomyocytes.
  • Reduces batch variability by optimizing cell recovery conditions.

Instructions for Use

Cell Isolation Protocol:

1. Tissue Preparation & Digestion:
   • Wash dissected heart tissue in HBSS (without Ca²⁺, Mg²⁺) before enzymatic digestion.
   • Digest tissue using Cardiomyocyte Isolation Enzymes 1 & 2.
2. Cell Washing & Collection:
   • Centrifuge and resuspend isolated cells in HBSS (without Ca²⁺, Mg²⁺).
   • Wash cells twice to remove enzyme contaminants.
3. Cell Culture & Assay Preparation:
   • Resuspend washed cells in DMEM supplemented with Cardiomyocyte Growth Supplements.
   • Plate cells in culture vessels for further differentiation or functional assays.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 4°C, protected from light.
  • Do not freeze, as freezing may alter buffer composition.
• Handling Precautions:
  • Use aseptic techniques to prevent contamination.
  • Ensure proper CO₂ incubation (5–10%) if using in culture media preparation.
• Disposal Guidelines:
  • Dispose of used buffer and reagents in accordance with laboratory waste management protocols.
  • If used with biohazardous samples, autoclave before disposal.

Why Choose Thermo Scientific™ Hanks' Balanced Salt Solution (HBSS) (No Ca²⁺, Mg²⁺)?

✅ Optimized for cardiomyocyte isolation, reducing processing time to <2 hours

✅ Enhances cell yield (1.5–4x) and viability compared to traditional methods

✅ Prevents premature cell attachment during primary cell washing

✅ Sterile, ready-to-use formulation for consistent experimental outcomes

✅ Compatible with enzymatic dissociation methods for cardiac cell isolation

Thermo Scientific™ Hanks' Balanced Salt Solution (HBSS) (No Ca²⁺, Mg²⁺) is an essential buffer for primary cardiomyocyte isolation, functional assays, and cell culture workflows, ensuring high yield and viability for cardiac research applications.