0
4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (Pnpp) ≥98% Analytical Reagent, 5G
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | molecular biology, Colorimetric Assay, ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | VWR | ||
| Product Grade | Analytical grade | Formula | C₆H₄NNa₂O₆P·6H₂O |
High-Purity Chromogenic Substrate for Phosphatase Assays
VWR 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (pNPP) ≥98% is a high-purity chromogenic substrate widely used for the determination of acid and alkaline phosphatase activity in biochemical, clinical, and research applications. Upon enzymatic hydrolysis, pNPP produces a yellow reaction product that absorbs light at 405 nm, facilitating quantitative spectrophotometric analysis.
Key Features & Benefits
✅ High Purity (≥98%) – Ensures precision and reproducibility in phosphatase assays.
✅ Chromogenic Detection – Produces a yellow chromophore at 405 nm for easy spectrophotometric measurement.
✅ Analytical Grade Reagent – Suitable for biochemical assays, enzymology studies, and research applications.
✅ Stable & Reliable – Maintains high stability when stored properly.
✅ Convenient Packaging – Available in a plastic bottle for solids for easy handling.
Chemical & Physical Properties
| Property | Details |
|---|---|
| Synonyms | 4-Nitrophenyl phosphate disodium salt hexahydrate, Phosphatase substrate, p-Nitrophenyl phosphate, Phosphoric acid 4-nitrophenyl ester disodium salt hexahydrate |
| Chemical Formula | C₆H₄NNa₂O₆P·6H₂O |
| Molecular Weight | 371.144 g/mol |
| Melting Point | >300 °C |
| Appearance | White to off-white crystalline powder |
| Solubility | Soluble in water |
| Absorbance Maximum | 405 nm |
| Storage Conditions | Freezer (-20°C recommended) |
| CAS Number | 333338-18-4 |
| EINECS Number | 224-246-5 |
| MDL Number | MFCD00066288 |
Specification & Quality Control
| Specification | Test Results |
|---|---|
| Assay (Purity) | Min. 98% |
| 4-Nitrophenol Content | Max. 0.2% |
| Water Content | Max. 30% |
| Iron (Fe) Content | Max. 20 ppm |
Available Formats & Catalog Numbers
| Pack Type | Quantity | Catalog Number |
|---|---|---|
| Plastic bottle for solids | 5 g | 27963.101 |
Applications & Use Cases
🔬 Enzymatic Assays – Used for alkaline and acid phosphatase determination in biochemical studies.
🔬 Clinical & Pharmaceutical Research – Supports enzyme activity measurement in diagnostic and drug discovery research.
🔬 Biochemical Studies – Applied in enzyme kinetics, molecular biology, and cellular assays.
🔬 Spectrophotometric Analysis – Ideal for colorimetric detection of phosphatase activity at 405 nm.
🔬 Food & Environmental Testing – Used for contaminant and enzyme activity assessment in various industries.
Handling & Storage
- Store at -20°C (freezer) to maintain chemical stability.
- Protect from moisture and light to prevent degradation.
- Use appropriate safety precautions when handling reagents.
- For Research Use Only – Not intended for diagnostic or therapeutic applications.
VWR 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (pNPP) ≥98% is a high-quality analytical reagent designed for precise phosphatase determination in research and biochemical applications. Its high purity, stability, and chromogenic properties make it an essential reagent for enzyme assays, clinical research, and molecular biology studies.
For bulk orders, pricing inquiries, or technical support, please contact LabMart Limited. We are dedicated to providing high-quality reagents for your research needs.