Thermo Scientific™ 4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) 97+%

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Specifications:
Application molecular biology, Colorimetric Assay, ELISA-Based Assays
Storage Temperature 2-8°C
Product Type Molecular Biology Reagent Forms Powder
Product Brand Thermo Fisher Scientific™
Product Grade Molecular Biology Formula C₆H₄NNa₂O₆P·6H₂O

High-Purity Chromogenic Substrate for Phosphatase Assays 

Thermo Scientific™ 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (pNPP) is a high-quality chromogenic substrate used for the determination of acid and alkaline phosphatases in biochemical and clinical research applications. This reagent produces a yellow color upon hydrolysis by phosphatases, allowing for spectrophotometric detection and quantification.

Key Features & Benefits:

High Purity (97+%)

  • Ensures reliable and reproducible enzymatic assay results.
  • Suitable for high-sensitivity phosphatase activity measurements.

Chromogenic Phosphatase Substrate

  • Produces a yellow reaction product upon hydrolysis.
  • Enables spectrophotometric detection at 405 nm.

Versatile Enzymatic Substrate

  • Suitable for both alkaline and acid phosphatase assays.
  • Widely used in biochemical, clinical, and life sciences research.

Stable and Easy to Handle

  • Long shelf-life when stored under recommended conditions.
  • Available in convenient packaging sizes.

Chemical & Physical Properties:

FeatureDetails
Chemical FormulaC₆H₄NNa₂O₆P·6H₂O
Molecular Weight371.144 g/mol
Melting Point>300 °C
Storage Temperature2–8°C (Refrigerated)
MDL NumberMFCD00066288
CAS Number333338-18-4
EINECS Number224-246-5

Available Formats & Catalog Numbers:

Pack TypeQuantityCatalog Number
Glass bottle (solid)5 gA12310.06
Glass bottle (solid)25 gA12310.14
Glass bottle (solid)100 gA12310.22

Recommended Applications:

  • Enzyme activity assays for acid and alkaline phosphatases
  • ELISA and colorimetric detection methods
  • Clinical diagnostics and biochemical research
  • Molecular biology and life sciences studies

Thermo Scientific™ 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (pNPP) is a high-purity chromogenic substrate essential for phosphatase detection in research and diagnostic applications. Its high sensitivity and stability make it a preferred choice for enzyme assays and colorimetric detection techniques.

For product inquiries, bulk orders, or technical assistance, please contact LabMart Limited. We are committed to providing high-quality laboratory reagents for your research success!

  • Pack Size:   100g 5g 25g
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