0
Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The 1-Step™ TMB ELISA Substrate Solutions by Thermo Scientific™ are ready-to-use, non-carcinogenic substrates for detecting horseradish peroxidase (HRP) activity in ELISA assays. These substrates yield a blue color upon oxidation by HRP, which shifts to yellow upon the addition of a stop solution (sulfuric or phosphoric acid).
Key Features:
- Versatile Sensitivities:
- Available in three formulations:
- 1-Step Slow TMB: Intermediate sensitivity.
- 1-Step Turbo TMB: High sensitivity, comparable to OPD at ~1 mg/mL.
- 1-Step Ultra TMB-ELISA: Highest sensitivity with a signal-to-noise ratio in the picogram range.
- Available in three formulations:
- Colorimetric Detection:
- Initial blue color (Amax = 370 nm and 652 nm).
- Converts to yellow upon stopping the reaction (Amax = 450 nm).
- Enhanced Performance:
- Faster oxidation than other HRP substrates.
- Free of DMF (dimethylformamide) or DMSO (dimethyl sulfoxide).
- User-Friendly:
- Ready-to-use with no additional preparation or filtering.
- Suitable for kinetic and endpoint ELISA assays.
- Non-Carcinogenic:
- Safer for laboratory use compared to traditional HRP substrates.
Specifications:
| Attribute | Details |
|---|---|
| Product Line | 1-Step™ |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Detection Method | Colorimetric |
| Substrate Properties | Chemical substrate |
| Unit Sizes Available | 250 mL, 400 mL, and 1 L |
| Shipping Condition | Ambient temperature |
| Storage Condition | Store at 4°C, protected from moisture. |
Product Variants:
| Catalog Number | Product Description | Volume |
|---|---|---|
| 34028 | 1-Step Ultra TMB-ELISA Substrate Solution | 250 mL |
| 34024 | 1-Step Slow TMB-ELISA Substrate Solution | 250 mL |
| 34022 | 1-Step Turbo TMB-ELISA Substrate Solution | 250 mL |
| 34029 | 1-Step Ultra TMB-ELISA Substrate Solution | 1 L |
| 34021 | TMB Substrate Kit | 400 mL |
Applications:
- Quantitative and qualitative ELISA.
- Kinetic or endpoint assays.
- Gene expression, immunology, and diagnostic research.
Advantages:
- Custom Sensitivity Options: Choose the formulation best suited to your assay requirements.
- Ready-to-Use: Simplifies workflows and minimizes preparation errors.
- High Signal-to-Noise Ratio: Ensures reliable and reproducible results.
- Safety: Non-carcinogenic and free of DMF/DMSO, making it safer for routine laboratory use.
Comparison of Sensitivity Levels:
| Substrate | Sensitivity | Recommended Use |
|---|---|---|
| 1-Step Slow TMB | Intermediate sensitivity (more sensitive than ABTS, less sensitive than Turbo TMB or OPD). | Kinetic assays. |
| 1-Step Turbo TMB | Comparable to OPD (1 mg/mL); high sensitivity. | Routine assays needing high signals. |
| 1-Step Ultra TMB | Highest sensitivity; excellent signal-to-noise ratio in the picogram range. | Low-abundance target detection. |
Storage and Handling:
- Store at 4°C upon receipt.
- Protect from moisture and direct light for optimal stability.
References:
- Josephy, P.D., et al. (1982). J. Biol. Chem. 257(7), 3669-3675.
- Josephy, P.D., et al. (1983). J. Biol. Chem. 258(9), 5561-5569.
- Liem, H.H., et al. (1979). Anal. Biochem. 98, 388-393.
- Substrate and Size: 1-Step Ultra TMB-ELISA Substrate Solution, 250 mL 1-Step Slow TMB-Elisa Substrate Solution, 250 mL 1-Step Turbo TMB-ELISA Substrate Solution, 250 mL 1-Step Ultra TMB-ELISA Substrate Solution, 1 L TMB Substrate Kit, 400 mL
Add a Review
Customer Review
Showing
0
Of
0