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Virplex® Bacterial Meningitis RealTime PCR Kit, 6-Plex, 48 Rxn
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Target Pathogens: Streptococcus pneumoniae (SPN), Listeria monocytogenes (LMO), Streptococcus agalactiae (SAG), Haemophilus influenzae (HIN), Neisseria meningitidis (NME), Escherichia coli K1 (ECOK1)
Specifications:
| Application | qPCR, Molecular Diagnostics | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Lyophilized |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® Bacterial Meningitis RealTime PCR Kit is a multiplex real-time PCR assay designed for the detection of nucleic acids from six key bacterial pathogens causing meningitis in human cerebrospinal fluid (CSF) samples. This one-step amplification kit provides rapid, sensitive, and reliable results, ensuring effective laboratory support for bacterial meningitis research and outbreak investigations.
Key Features
- 7-target multiplex PCR – two reaction tubes per sample for optimal detection.
- Simultaneous analysis of six pathogens – Streptococcus pneumoniae (SPN), Listeria monocytogenes (LMO), Streptococcus agalactiae (SAG), Haemophilus influenzae (HIN), Neisseria meningitidis (NME), and Escherichia coli K1 (ECOK1).
- Internal control – monitors amplification inhibition and validates correct reaction set-up.
- Compatible with 4-channel qPCR cyclers – FAM, HEX (VIC), Texas Red (ROX), and Cy5.
- Fast results – under 2 hours.
- Lyophilized reagents – master mix and positive control ensure stability and reduce transportation costs.
- Flexible formats – available in vials or pre-dispensed strip-divisible plates for user convenience.
- Research Use Only (RUO) – intended for laboratory and research applications.
Applications
- Detection of bacterial meningitis pathogens in human CSF samples.
- Epidemiological research and surveillance of meningitis outbreaks.
- Validation and development of multiplex molecular assays.
- Training and competency assessment in molecular diagnostics.
- Quality assurance in clinical microbiology research labs.
Product Details
| Property | Value |
|---|---|
| Product Name | Virplex® Bacterial Meningitis RealTime PCR Kit |
| Reference | RTPCR014-LPD-R |
| Nº Tests | 48 |
| Class | RUO – Research Use Only |
| Sample Type | Cerebrospinal fluid (CSF) |
| Pathogens Detected | Streptococcus pneumoniae (SPN), Listeria monocytogenes (LMO), Streptococcus agalactiae (SAG), Haemophilus influenzae (HIN), Neisseria meningitidis (NME), Escherichia coli K1 (ECOK1) |
| Targets | 7 total (6 bacterial + 1 internal control) |
| PCR Cycler Compatibility | FAM, HEX (VIC), Texas Red (ROX), Cy5 |
| Time to Results | < 2 hours |
| Formats | Vials and pre-dispensed strip-divisible plates |
| Reagents | Lyophilized master mix, positive control, negative control |
| Target | Pathogen / Marker | Clinical Relevance |
|---|
| 1 | Streptococcus pneumoniae (SPN) | Major cause of bacterial meningitis, pneumonia, and sepsis, especially in children and the elderly. |
| 2 | Listeria monocytogenes (LMO) | Causes listeriosis and meningitis in neonates, pregnant women, and immunocompromised patients. |
| 3 | Streptococcus agalactiae (SAG, Group B Strep) | Leading cause of neonatal meningitis and sepsis. |
| 4 | Haemophilus influenzae (HIN) | Historically a major cause of pediatric meningitis; incidence reduced due to Hib vaccination. |
| 5 | Neisseria meningitidis (NME) | Responsible for meningococcal disease and meningitis epidemics worldwide. |
| 6 | Escherichia coli K1 (ECOK1) | Common cause of neonatal meningitis, associated with high morbidity and mortality. |
| 7 | Internal Control (IC) | Monitors for PCR inhibition, nucleic acid integrity, and correct amplification setup. |
Virplex® Meningitis RealTime PCR Kits – Comparison
| Feature | Bacterial Meningitis Kit | Viral Meningitis Kit |
|---|---|---|
| Product Name | Virplex® Bacterial Meningitis RealTime PCR Kit | Virplex® Viral Meningitis RealTime PCR Kit |
| Reference | RTPCR014-LPD-R | RTPCR011 / RTPCR011-LPD |
| Nº Tests | 48 | 48 |
| Class | RUO (Research Use Only) | CE |
| Sample Type | Human cerebrospinal fluid (CSF) | Human cerebrospinal fluid (CSF) |
| Pathogens Detected | - Streptococcus pneumoniae (SPN) - Listeria monocytogenes (LMO) - Streptococcus agalactiae (SAG, GBS) - Haemophilus influenzae (HIN) - Neisseria meningitidis (NME) - Escherichia coli K1 (ECOK1) | - Herpes simplex virus 1 (HSV-1) - Herpes simplex virus 2 (HSV-2) - Varicella zoster virus (VZV) - Enteroviruses (EVs) - Human parechoviruses (HPeVs) - West Nile virus (WNV) - Toscana virus (TOSV) |
| Total Targets | 7 (6 pathogens + internal control) | 8 (7 pathogens + internal control) |
| Internal Control | DNA internal control | Bacteriophage MS2 RNA control |
| PCR Format | 2 tubes per sample | 2 tubes per sample |
| PCR Cycler Compatibility | 4-channel: FAM, HEX (VIC), Texas Red (ROX), Cy5 | 5-channel: FAM, HEX (VIC), Texas Red (ROX), Q705 (Cy5.5), Cy5 |
| Turnaround Time | < 2 hours | < 2 hours |
| Reagent Format | Lyophilized master mix & positive control | Lyophilized master mix A & B, positive/negative control |
| Kit Formats | Vials or pre-dispensed strip-divisible plate | Vials or pre-dispensed strip-divisible plate |
| Applications | Detection of major bacterial causes of meningitis in CSF samples | Detection of major viral causes of meningitis in CSF samples |
| Related AMPLIRUN® Controls | SPN, LMO, SAG, HIN, NME, ECOK1 DNA Controls | HSV-1, HSV-2, VZV, EVs, HPeVs, WNV, TOSV Controls + AMPLIRUN® Total Meningitis Control (CSF) |
Related Products
- Virplex® Viral Meningitis RealTime PCR Kit
- AMPLIRUN® Streptococcus agalactiae DNA Control
- AMPLIRUN® Streptococcus pneumoniae DNA Control
- AMPLIRUN® Escherichia coli DNA Control
- AMPLIRUN® Neisseria meningitidis DNA Control
- AMPLIRUN® Haemophilus influenzae DNA Control
- AMPLIRUN® Listeria DNA Control
The Virplex® Bacterial Meningitis RealTime PCR Kit provides a comprehensive molecular tool for the research detection of bacterial meningitis pathogens. With its multiplex design, rapid turnaround, and lyophilized format, it supports laboratories in outbreak research, epidemiology, and molecular microbiology quality control.