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Thermo Scientific™ Pierce™ Trifluoroacetic Acid (TFA), LC-MS Grade, 50 mL
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Specifications:
| Application | Protein Assays & Analysis, Mass Spectrometry | ||
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Analytical grade | Formula | C₂HF₃O₂ |
Thermo Scientific™ Pierce™ Trifluoroacetic Acid (TFA), LC-MS Grade is a high-purity ion-pairing reagent designed for reverse-phase HPLC and LC-MS peptide separation workflows. It is manufactured and tested to meet strict specifications for purity, clarity, and performance, helping support sensitive peptide detection, improved chromatographic resolution, and reproducible analytical results.
TFA is widely used as a mobile phase modifier in reverse-phase chromatography because it sharpens peptide peaks, improves separation resolution, is volatile, and has low UV absorbance at commonly used peptide detection wavelengths.
Key Features
| Feature | Description |
|---|---|
| High purity formulation | Supplied as 99.5% TFA for demanding analytical workflows |
| Ion-pairing reagent | Commonly used to improve reverse-phase HPLC peptide separations |
| Excellent clarity | Supports sensitive peptide detection at low UV wavelengths |
| Improved peak shape | Helps sharpen peptide peaks and enhance chromatographic resolution |
| Volatile reagent | Easily removed after chromatography or sample preparation |
| High-performance packaging | Packaged to help maintain TFA integrity and reduce contamination |
| Suitable for peptide workflows | Used in protein/peptide sequencing, amino acid analysis, and peptide synthesis |
Applications
| Application Area | Use |
|---|---|
| Reverse-phase HPLC | Ion-pairing reagent for protein and peptide separations |
| LC-MS workflows | Mobile phase modifier for peptide analysis |
| Protein and peptide sequencing | Supports preparation and separation of peptides |
| Protein/peptide solubilization | Used as a solubilizing agent in peptide workflows |
| Solid-phase peptide synthesis | Common reagent in peptide synthesis processes |
| Amino acid analysis | Suitable for analytical workflows involving amino acids and peptides |
| 0.1% TFA mobile phase preparation | Used to prepare reproducible TFA-containing chromatography solutions |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Thermo Scientific™ Pierce™ Trifluoroacetic Acid (TFA), LC-MS Grade |
| Catalog Number | 85183 |
| Item Name | Trifluoroacetic Acid (TFA) |
| Product Line | Pierce™ |
| Form | Liquid |
| Formulation | 99.5% TFA |
| Reagent Type | Ion Pairing Reagent |
| Quantity | 50 mL |
| Unit Size | Each |
| Storage | Store in a cool, dry, and well-ventilated area. Protect from direct sunlight |
| Use Statement | For Research Use Only. Not for use in diagnostic procedures |
0.1% TFA Solution Preparation Consideration
TFA is commonly used at 0.1% in reverse-phase peptide separations. For reproducibility, it is important to specify whether the concentration is prepared as:
| Concentration Format | Meaning |
|---|---|
| 0.1% w/v | 1 g of TFA per liter of mobile phase |
| 0.1% v/v | 1 mL of TFA per liter of mobile phase |
Because TFA has a density of approximately 1.53 g/mL, 0.1% w/v and 0.1% v/v solutions are not equivalent. Clearly defining the preparation method helps improve reproducibility between runs, laboratories, and published methods.
Related Product
| Product | Description |
|---|---|
| Pierce™ Trifluoroacetic Acid (TFA), Sequencing Grade | Related TFA reagent for peptide sequencing and reverse-phase HPLC workflows |
Thermo Scientific™ Pierce™ Trifluoroacetic Acid (TFA), LC-MS Grade is a high-purity liquid ion-pairing reagent for reverse-phase HPLC, LC-MS peptide analysis, protein/peptide sequencing, amino acid analysis, and peptide synthesis workflows. Its purity, clarity, volatility, and low UV absorbance make it a reliable mobile phase modifier for improving peptide peak shape, chromatographic resolution, and analytical reproducibility.