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Thermo Scientific™ EasyPep™ MS Sample Prep Kit
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Specifications:
| Application | Protein Assays & Analysis, Mass Spectrometry | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ EasyPep™ MS Sample Prep Kits are streamlined, ready-to-use sample preparation solutions designed for efficient, reproducible processing of protein samples for proteomic mass spectrometry analysis. The kits include optimized reagents and workflows for cell lysis, protein digestion, peptide clean-up, and preparation of MS-compatible peptide samples.
EasyPep™ kits are particularly useful for researchers working with cultured mammalian cells, tissues, plasma, and other protein-containing samples. The optimized workflow helps reduce sample variability, minimize hands-on time, and generate high-quality peptides suitable for direct MS analysis, TMT™ labeling, and other downstream proteomics applications.
Key Features
| Feature | Description |
|---|---|
| Complete workflow solution | Includes pre-formulated reagents for lysis, digestion, peptide clean-up, and MS sample preparation |
| Fast processing | Reduces sample preparation time from more than one day to less than three hours |
| Reproducible results | Standardized reagents and optimized protocol help reduce sample-to-sample variability |
| Flexible sample input | Suitable for protein samples from 10–100 µg |
| Broad sample compatibility | Verified for use with cells, plasma, and tissue samples |
| Efficient digestion | Uses trypsin/Lys-C protease mix for improved protein digestion |
| Reduced viscosity | Universal Nuclease helps reduce nucleic acid-related viscosity without sonication |
| MS-compatible output | Produces detergent-free peptide samples suitable for direct mass spectrometry analysis |
| TMT™ compatible | Prepared peptides are compatible with TMT labeling and other downstream proteomics workflows |
Ordering Information
| Catalog Number | Description | Size | Workflow Step |
|---|---|---|---|
| A40006 | EasyPep™ Mini MS Sample Prep Kit | 20 reactions | Cell lysis |
| A57864 | EasyPep™ Micro MS Sample Prep Kit | 96 reactions | Mass spectrometry sample preparation |
| A45733 | EasyPep™ MS Sample Prep Kit | 96 reactions | Protein digestion |
| A45734 | EasyPep™ Maxi Sample Prep Kit | 8 reactions | Protein digestion |
| A45735 | EasyPep™ Lysis Buffer | 100 mL | Cell lysis |
| A57865 | EasyPep™ Peptide Clean-up Plate | 96 reactions | Mass spectrometry sample preparation |
Applications
| Application Area | Use |
|---|---|
| Proteomic sample preparation | Preparation of peptide samples for LC-MS/MS and other MS workflows |
| Protein digestion | Conversion of protein samples into peptides using optimized enzyme mix |
| Cell and tissue proteomics | Processing of cultured mammalian cells and tissue samples |
| Plasma proteomics | Preparation of plasma-derived protein samples for MS analysis |
| Peptide clean-up | Removal of detergents and contaminants before mass spectrometry |
| TMT labeling workflows | Preparation of peptides suitable for isobaric labeling applications |
| Biomarker discovery research | Generation of reproducible peptide samples for comparative proteomics |
| Quantitative proteomics | Sample preparation for downstream quantitative MS analysis |
Workflow Overview
| Step | Description |
|---|---|
| 1. Sample lysis | Pre-formulated lysis reagents help efficiently extract proteins from samples |
| 2. Viscosity reduction | Universal Nuclease reduces nucleic acid-related viscosity, helping eliminate the need for sonication |
| 3. Reduction and alkylation | Rapid one-pot chemistry modifies cysteine residues through carbamidomethylation |
| 4. Protein digestion | Trypsin/Lys-C protease mix improves digestion efficiency and peptide generation |
| 5. Peptide clean-up | Clean-up columns or plates remove detergents and prepare peptides for MS analysis |
| 6. MS-ready peptide output | Final peptides are suitable for direct mass spectrometry or downstream labeling workflows |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Line | EasyPep™ |
| Product Type | Sample Preparation Kit |
| Final Product Type | Peptides |
| Detection Method | Mass Spectrometry |
| For Use With | Mass Spectrometer |
| Starting Material | Protein Samples |
| Recommended Sample Input | 10–100 µg protein |
| Workflow Compatibility | Cell lysis, protein digestion, peptide clean-up, MS sample preparation |
| Downstream Compatibility | Direct MS analysis, TMT™ labeling, proteomics workflows |
| Shipping Condition | Wet ice |
| Unit Size | Each |
| Use Statement | For Research Use Only. Not for use in diagnostic procedures |
Unique Advantages
| Advantage | Benefit |
|---|---|
| Less than three-hour workflow | Enables faster sample turnaround compared with traditional overnight digestion workflows |
| One-pot reduction and alkylation | Reduces handling steps and improves workflow consistency |
| Trypsin/Lys-C enzyme mix | Supports more complete digestion and reliable peptide generation |
| Universal Nuclease included | Reduces sample viscosity without mechanical disruption such as sonication |
| Integrated peptide clean-up | Produces detergent-free peptides suitable for high-quality MS analysis |
| Multiple kit formats | Mini, Micro, standard, and Maxi formats support different sample throughput needs |
Thermo Scientific™ EasyPep™ MS Sample Prep Kits provide an efficient, reproducible, and time-saving solution for preparing peptide samples for proteomic mass spectrometry analysis. With optimized reagents for lysis, digestion, reduction, alkylation, and peptide clean-up, the EasyPep™ workflow helps researchers generate high-quality MS-compatible peptide samples in less than three hours. The availability of Mini, Micro, standard, Maxi, lysis buffer, and peptide clean-up formats makes the product line suitable for a wide range of proteomics research workflows, from small-scale sample preparation to higher-throughput MS applications.
- Type: EasyPep™ Mini MS Sample Prep Kit, 20 reactions EasyPep™ Micro MS Sample Prep Kit, 96 reactions EasyPep™ MS Sample Prep Kit, 96 reactions EasyPep™ Maxi Sample Prep Kit, 8 reactions EasyPep™ Lysis Buffer, 100 mL EasyPep™ Peptide Clean-up Plate, 96 reactions