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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Grade | Molecular Biology | ||
The Human Immunoglobulin D (IgD) ELISA Kit is designed for the quantitative detection of human IgD concentrations in serum, plasma, culture supernatants, and other biological fluids.
This sandwich enzyme-linked immunosorbent assay (ELISA) provides high sensitivity and specificity for human IgD, enabling precise evaluation of humoral immune status in research studies.
The kit employs pre-coated microplate wells with an IgD-specific capture antibody and a Horseradish Peroxidase (HRP)-conjugated detection antibody, allowing accurate quantification through optical density (OD) measurement at 450 nm.
Key Features
- High Sensitivity: Detection limit as low as 0.06 ng/mL with an assay range of 0.3–20 ng/mL.
- Accurate and Reproducible: Intra-assay CV < 10%, inter-assay CV < 12%.
- Flexible Format: Available in 96-test and 48-test configurations.
- Ready-to-Use Reagents: Includes all buffers, standards, and reagents required for complete assay performance.
- Wide Sample Compatibility: Suitable for serum, plasma, tissue homogenate, culture media, and other biological fluids.
- Validated Procedure: Based on standard sandwich ELISA principle with HRP/TMB colorimetric detection.
- Quality Control: Each kit lot is validated for sensitivity, linearity, and precision.
Principle of the Assay
This kit utilizes a sandwich-ELISA principle:
- Microplate wells pre-coated with an anti-human IgD antibody capture IgD in the sample.
- After washing, an HRP-conjugated secondary antibody binds to the captured IgD.
- Following substrate addition (TMB), color develops proportionally to the IgD concentration.
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The reaction is terminated, and OD is measured at 450 nm.
The sample concentration is determined by comparing OD values to a standard calibration curve.
Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Sandwich ELISA (Colorimetric, 450 nm) |
| Assay Range | 0.3 – 20 ng/mL |
| Sensitivity | 0.06 ng/mL |
| Precision | Intra-assay CV < 10%; Inter-assay CV < 12% |
| Sample Types | Serum, plasma, cell culture supernatant, tissue extract, urine, cerebrospinal fluid |
| Storage Temperature | 2 – 8 °C |
| Shelf Life | 6 months |
| Total Assay Time | Approx. 3 hours |
| Sample Volume Required | 10 µL per well |
| Detection Antibody | HRP-conjugated anti-human IgD |
| Stop Solution | Acidic stop reagent (included) |
| Standard Concentration | 27 ng/mL standard provided (dilution series for curve generation) |
Kit Components
| Component | Description | Storage |
|---|---|---|
| Microelisa Strip Plate | Pre-coated with anti-human IgD antibody | 2–8 °C |
| Standard (27 ng/mL) | 0.5 mL vial | 2–8 °C |
| Standard Diluent | 1.5 mL | 2–8 °C |
| HRP-Conjugate Reagent | 6 mL | 2–8 °C |
| Sample Diluent | 6 mL | 2–8 °C |
| Chromogen Solutions A & B | 6 mL each | 2–8 °C |
| Stop Solution | 6 mL | 2–8 °C |
| Wash Solution (30×) | 20 mL concentrate | 2–8 °C |
| User Manual, Closure Membranes, Plate Sealers | Included | Room Temp |
Applications
- Quantitative analysis of human IgD levels in serum or plasma.
- Immunological research on humoral immune responses and B-cell activation.
- Monitoring IgD expression in autoimmune and inflammatory diseases.
- Evaluation of monoclonal antibody production and immune regulation.
- Suitable for basic research, cell culture analysis, and diagnostic development (RUO).
Safety and Regulatory Information
- Intended Use: For Research Use Only (RUO); not for clinical or diagnostic purposes.
- Storage: 2–8 °C. Avoid freeze–thaw cycles.
- Stability: Six months from manufacturing date under recommended conditions.
- Safety Note: Handle all human-derived materials as potentially infectious.
The Human Immunoglobulin D (IgD) ELISA Kit provides a sensitive and accurate assay for the quantification of human IgD in biological samples.
With its validated reagents, precise standard curve, and user-friendly format, this kit ensures reproducible results for immunological, clinical, and molecular biology research applications.
- Pack Size: For 48 Tests For 96 Tests