Human Immunoglobulin Free Light Chains Kappa (FLC-IgK) ELISA Kit

The Human Immunoglobulin Free Light Chains Kappa (FLC-IgK) ELISA Kit is a high-sensitivity sandwich ELISA designed for the quantitative measurement of free kappa light chains (FLC-IgK) in human biological fluids. Optimized for use in serum, plasma, urine, cerebrospinal fluid, pleural/peritoneal fluid, cell culture supernatants, and tissue homogenates, this kit is a trusted solution in multiple myeloma, immunology, oncology, and autoimmune disease research.

Key Features

• High Sensitivity – Detects as low as 0.05 pg/mL.
• Broad Dynamic Range – 0.3 – 20 pg/mL.
• Reliable & Reproducible – Intra-assay CV < 10%, Inter-assay CV < 12%.
• Flexible Pack Sizes – Available in 48T and 96T to suit small or large projects.
• Compatible with Multiple Sample Types – Serum, plasma, urine, CSF, pleural/peritoneal fluid, tissue homogenates, and culture supernatants.
• Complete Kit – All required reagents included, ready-to-use.
• Research Grade – For research use only (RUO), not for diagnostic use.

Kit Contents

• Pre-coated MicroELISA Strip Plate (48 or 96 wells depending on kit size)
• Standards (27 pg/mL) & Standard Diluent
• HRP-Conjugate Reagent
• Sample Diluent
• Chromogen Solutions A & B
• Stop Solution
• Wash Buffer (Concentrate)
• Closure Plate Membrane
• Sealed Storage Bag
• User Manual

(Reagent volumes are scaled according to 48T or 96T format.)

Technical Specifications

Applications

• Multiple Myeloma Research – Quantifying free kappa chains for disease monitoring.
• B-cell Malignancies – Assessing abnormal immunoglobulin secretion.
• Autoimmune & Immunodeficiency Disorders – Evaluating free light chain dysregulation.
• Clinical Research Studies – Investigating immune system and kidney function biomarkers.

Storage & Handling

• Store reagents at 2–8 °C.
• Shelf life: 6 months.
• Avoid repeated freeze–thaw cycles.
• Do not use with samples containing sodium azide (NaN₃).

Ordering Information

The Human Immunoglobulin Free Light Chains Kappa (FLC-IgK) ELISA Kit provides a sensitive, accurate, and reproducible solution for detecting free kappa light chains in human samples. With its broad sample compatibility, consistent results, and availability in both 48-test and 96-test formats, this kit is ideal for researchers investigating immunoglobulin disorders, multiple myeloma, and immune system regulation. It ensures confidence in data quality while offering flexibility for both small-scale studies and larger projects.