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Thermo Scientific™ Doxorubicin Hydrochloride ≥97%
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Anthracycline Antibiotic | Topoisomerase II Inhibitor | Antineoplastic Agent
Specifications:
| Application | cell Signalling, Apoptosis | ||
| Storage Temperature | -20°C | ||
| Product Type | Biochemical Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₂₇H₃₀ClNO₁₁ |
Thermo Scientific™ Doxorubicin Hydrochloride (≥97%) is a naturally derived anthracycline antibiotic with potent antitumor and cytotoxic properties, originally isolated from Streptomyces peucetius.
It exerts its effect through DNA intercalation and topoisomerase II inhibition, preventing DNA replication and transcription. This process triggers apoptosis and oxidative stress via free radical formation, making doxorubicin one of the most studied agents in cancer and drug-resistance research.
Its inherent fluorescent properties make it useful for studying drug efflux pumps, subcellular trafficking, and organelle sequestration (Golgi and lysosomal systems).
Key Features
- High Purity: ≥97% confirmed by supplier’s CoA.
- Mechanism of Action: DNA intercalator and topoisomerase II inhibitor.
- Dual Activity: Inhibits nucleic acid synthesis and induces apoptosis.
- Fluorescent Compound: Enables real-time visualization of cellular uptake and efflux.
- Biologically Active: Generates reactive oxygen species, leading to lipid peroxidation and cell death.
- Research-Grade Quality: Ideal for mechanistic, biochemical, and cytotoxicity assays.
- Storage: Store in a cool, dry place away from light and oxidizers.
- Research Use Only (RUO): Not for therapeutic or diagnostic applications.
Chemical Identifiers
| Property | Value |
|---|---|
| IUPAC Name | Hydrogen (8S,10S)-10-[(4-amino-5-hydroxy-6-methyloxan-2-yl)oxy]-6,8,11-trihydroxy-8-(2-hydroxyacetyl)-1-methoxy-5,7,8,9,10,12-hexahydrotetracene-5,12-dione chloride |
| Synonyms | Adriamycin hydrochloride, Dox hydrochloride, Hydrochloride of Doxorubicin |
| CAS Number | 25316-40-9 |
| Molecular Formula | C₂₇H₃₀ClNO₁₁ |
| Molecular Weight | 579.98 g/mol |
| InChI Key | MWWSFMDVAYGXBV-FGBJBKNOSA-N |
| SMILES | [H+].[Cl-].COC1=CC=CC2=C1C(=O)C1=C(O)C3=C(CC@(C[C@@H]3OC3CC(N)C(O)C(C)O3)C(=O)CO)C(O)=C1C2=O |
Specifications
| Parameter | Specification |
|---|---|
| Purity (HPLC) | ≥97% |
| Form | Crystalline or granular powder |
| Color | Red to orange |
| Mechanism of Action | DNA intercalation and topoisomerase II inhibition |
| Solubility | Soluble in water, DMSO, and methanol |
| Storage Conditions | Store in a cool, dry, well-ventilated place |
| Packaging | Glass bottle |
| Intended Use | Research Use Only (RUO) |
Applications
- Oncology Research: Investigation of cytotoxicity, apoptosis, and DNA repair pathways.
- Cell Biology: Visualization of drug transport and efflux mechanisms via fluorescence.
- Pharmacology: Mechanistic studies of topoisomerase II inhibitors and anthracycline activity.
- Drug Delivery Research: Use in nanoparticle, liposome, and polymeric carrier systems (e.g., PLGA-based).
- Resistance Studies: Development of doxorubicin-resistant HepG2 and K562 cell lines.
- Toxicology: Analysis of oxidative stress and lipid peroxidation.
Safety and Handling
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Classification (EC 1272/2008):
- Acute Toxicity (Oral) Cat. 4 | Eye Damage Cat. 1 | Mutagenicity Cat. 1A | Reproductive Toxicity Cat. 2
- Signal Word: Danger
- Hazard Statements: H302, H315, H318, H335, H340, H361fd
- Precautions: Use appropriate PPE; avoid inhalation and skin contact; restricted to professional users.
References
- Benival, D.M. & Devarajan, P.V. “In Situ Lipidization for SMEDDS Design of Doxorubicin Hydrochloride.” J. Biomed. Nanotechnol. (2015) 11(5), 913–922.
- Esmaeili, A. & Hadad, N.A. “ZnFe₂O₄-Chitosan-Doxorubicin Nanoparticles for Hyperthermic Therapy.” Ceram. Int. (2015) 41(6), 7529–7535.
The Thermo Scientific™ Doxorubicin Hydrochloride is a research-grade anthracycline antibiotic and topoisomerase II inhibitor used for DNA intercalation, apoptosis induction, and fluorescence-based cell transport studies.
With high purity and consistent performance, it is indispensable in oncology, drug delivery, and mechanistic pharmacology research.
- Pack Size: 10mg 50mg