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Gibco™ B-27™ Supplement (50X), minus vitamin A
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Specifications:
| Application | Cell culture, neural stem cell culture | ||
| Storage Temperature | -20°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
The Gibco™ B-27™ Supplement (50X), Minus Vitamin A is a variant of the original serum-free B-27™ formulation, optimized for the growth and proliferation of neural stem cells (NSCs) and neural progenitor cells (NPCs).
By excluding vitamin A (retinyl acetate/retinol), this supplement prevents the differentiation-inducing effects of retinoic acid, maintaining NSCs in an undifferentiated and proliferative state.
Based on the trusted B-27™ formulation, this serum-free supplement ensures reproducibility, consistency, and optimal performance in neurosphere and adherent monolayer cultures.
Key Features
- Vitamin A–free formulation: Prevents retinoic acid–induced differentiation of NSCs.
- Optimized for stem cell expansion: Supports proliferation and self-renewal of embryonic and adult neural stem/progenitor cells.
- Versatile use: Suitable for both neurosphere suspension cultures and adherent monolayer systems.
- Proven reliability: Derived from the most cited and validated B-27™ neuronal supplement.
- High quality manufacturing: Produced in a cGMP-compliant, ISO 13485-certified facility.
- Comprehensive quality testing: Each lot validated for performance, sterility, endotoxin, and mycoplasma.
- Storage: −5 to −20 °C; protect from light.
- Shelf Life: 12 months.
- Research Use Only (RUO): Not intended for diagnostic or therapeutic applications.
Specifications
| Parameter | Specification |
|---|---|
| Cell Type | Neural stem cells, neural progenitor cells |
| Concentration | 50X |
| Culture Type | Mammalian cell culture |
| Product Line | B-27™ |
| Serum Level | Serum-free |
| Form | Liquid |
| Sterility | Sterile |
| Tested For | Endotoxin, performance, sterility, mycoplasma |
| Quantity | 10 mL or 100 mL |
| Shelf Life | 12 months |
| Storage Conditions | −5 to −20 °C; protect from light |
| Shipping Conditions | Dry ice |
| Intended Use | Research Use Only (RUO) |
Applications
- Neural stem and progenitor cell culture under serum-free conditions.
- NSC proliferation and maintenance without retinoic acid–induced differentiation.
- Neurosphere and adherent monolayer culture systems.
- Pluripotent stem cell differentiation to neural precursors in defined media.
- Neurogenesis and neural development research.
- CNS and PNS model development in serum-free media.
The Gibco™ B-27™ Supplement (50X), Minus Vitamin A provides a defined, serum-free environment optimized for maintaining neural stem and progenitor cells in their undifferentiated state.
Manufactured to cGMP and ISO 13485 standards, it delivers the same performance, consistency, and reliability as the original B-27™, while enabling precise control over retinoic acid–dependent signaling during neurodevelopmental and stem cell research.
- Pack Size: 100 mL 10 mL