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Specifications:
| Application | Cell culture, neural stem cell culture | ||
| Storage Temperature | -20°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
The Gibco™ N-2 Supplement (100X) is a chemically defined, serum-free supplement that supports the growth, viability, and differentiation of neural and other mammalian cell types.
Based on Bottenstein’s N-1 formulation, the N-2 Supplement provides optimized concentrations of insulin, iron-rich holo-transferrin, progesterone, putrescine, and sodium selenite—components essential for neuronal health and proliferation.
It is widely used in neural stem cell culture, neuroblastoma cell maintenance, and pluripotent stem cell differentiation into neural or pancreatic-like lineages. The supplement performs optimally when used with Gibco™ Neurobasal™ Medium, DMEM, or serum-containing systems for astrocyte culture.
Key Features
- Chemically Defined & Serum-Free: Ensures consistency and reproducibility across experiments.
- Complete Formulation: Contains insulin, holo-transferrin, progesterone, putrescine, and selenite.
- Versatile Applications: Supports CNS, PNS, neuroblastoma, and astrocyte cultures.
- Performance Tested: Each lot validated for sterility, endotoxin levels, and culture performance.
- Stable Concentrate: Provided as a 100X liquid formulation for easy supplementation.
- cGMP Manufactured: Produced in an ISO 13485-certified, FDA-registered facility for consistent quality.
- Storage: −5 to −20 °C; protect from light.
- Shelf Life: 18 months from date of manufacture.
- Research Use Only (RUO): Not for diagnostic or therapeutic applications.
Specifications
| Parameter | Specification |
|---|---|
| Cell Type | Neural cells |
| Concentration | 100X |
| Culture Type | Mammalian cell culture |
| Serum Level | Serum-free |
| Form | Liquid |
| Sterility | Sterile |
| pH | 6–8 |
| Quantity | 5 mL or 50 mL |
| Shelf Life | 18 months |
| Storage Conditions | −5 to −20 °C; protect from light |
| Shipping Conditions | Dry ice |
| Tested For | Sterility, endotoxin, performance |
| Intended Use | Research Use Only (RUO) |
Applications
- Neural Stem Cell Culture: Maintenance and expansion of CNS and PNS cells.
- Neuroblastoma and Neuronal Lines: Proven compatibility with SH-SY5Y, SK-N-SH, and HT22 cells.
- Pluripotent Stem Cell Differentiation: Promotes neural and pancreatic-like cell development.
- Astrocyte Culture: Effective with DMEM and low FBS levels for astrocytic growth.
- Co-culture Systems: Supports primary neurons in mixed neural-glial cultures.
Summary
The Gibco™ N-2 Supplement (100X) provides a reliable, defined formulation for serum-free neuronal and mammalian cell culture.
Its proven performance and flexibility make it indispensable for neurobiology, stem cell differentiation, and neural model research. Manufactured under cGMP and ISO 13485 standards, it ensures consistent, high-quality results trusted by researchers worldwide.
- Pack Size: 50 mL 5 mL