Thermo Scientific™ ActivX™ Desthiobiotin-FP Serine Hydrolase Probe, 4.6 μg

Thermo Scientific™ ActivX™ Desthiobiotin-FP Serine Hydrolase Probe, 4.6 μg

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Specifications:

Application	Protein Assays & Analysis, Mass Spectrometry, Chemiluminescent Western Blot Imaging
Storage Temperature	–80°C
Product Type	Proteins & Peptides	Forms	Lyophilized
Product Brand	Thermo Fisher Scientific™
Product Grade	Molecular Biology

Overview
Resource

Thermo Scientific™ ActivX™ Desthiobiotin-FP Serine Hydrolase Probe is an activity-based enrichment probe designed for selective labeling and profiling of enzymatically active serine hydrolases. The probe contains a reactive fluorophosphonate (FP) group that covalently labels the active-site serine residue of functional serine hydrolases, together with a desthiobiotin tag that enables selective enrichment for downstream analysis.

This probe is useful for studying active enzyme levels, mapping active-site serine residues, profiling serine hydrolase activity, and evaluating small molecule inhibitor specificity and affinity. It supports workflows such as pull-down enrichment, Western blotting, chemiluminescent detection, and LC-MS/MS-based proteomics.

Key Features

Feature	Details
Activity-Based Probe	Selectively labels active serine hydrolases
Fluorophosphonate Reactive Group	Covalently targets the active-site serine of enzymatically active hydrolases
Desthiobiotin Tag	Enables selective enrichment of labeled proteins or peptides
Functional Enzyme Profiling	Detects biologically active enzymes at the time of labeling
Inhibitor Screening Support	Useful for assessing inhibitor binding, specificity, and off-target effects
Multiple Detection Workflows	Compatible with Western blot, chemiluminescence, and mass spectrometry workflows
Broad Enzyme Coverage	Suitable for proteases, lipases, esterases, amidases, peptidases, and related serine hydrolases

Applications

ActivX™ Desthiobiotin-FP Serine Hydrolase Probe is suitable for:

Serine hydrolase activity profiling
Detection of active serine hydrolases in cells and lysates
Pull-down enrichment of active-site-labeled proteins
Mapping active-site serine residues
Profiling proteases, lipases, esterases, amidases, and peptidases
Small molecule inhibitor screening
Inhibitor target binding and specificity studies
Global target and off-target identification by LC-MS/MS
Western blot-based enzyme activity analysis
Activity-based protein profiling workflows

Product Specifications

Specification	Details
Product Name	ActivX™ Desthiobiotin-FP Serine Hydrolase Probe
Catalog Number	88317
Product Line	ActivX™
Type	Fluorophosphonate, FP probe
Target	Serine hydrolase
Substrate Type	Serine hydrolase substrate
Formulation	Desthiobiotin-fluorophosphonate
Molecular Weight	464
Label / Dye	Biotin and other haptens
Form	Lyophilized
Quantity	4.6 µg
Assay	Protein quantitation assay
Application	Pull-down
Detection Method	Chemiluminescence, mass spectrometry, Western blot
Unit Size	Each
Research Use	For Research Use Only; not for diagnostic procedures

Workflow Overview

Workflow	Description
Active-Site Labeling	FP reactive group covalently labels active-site serine residues in active serine hydrolases
Protein Enrichment	Desthiobiotin-labeled proteins can be enriched for SDS-PAGE and Western blot analysis
Peptide Enrichment	For MS workflows, labeled proteins are reduced, alkylated, digested, and enriched at the active-site peptide level
Inhibitor Profiling	Competitive labeling can be used to assess inhibitor target binding and off-target effects

Ordering Information

Catalog Number	Description	Quantity
88317	ActivX™ Desthiobiotin-FP Serine Hydrolase Probe	4.6 µg

Storage & Handling

Store the lyophilized probe at -80 °C, protected from light. Stock solutions prepared in DMSO can be stored at -20 °C for up to 6 months. Handle using appropriate laboratory personal protective equipment and avoid repeated freeze-thaw cycles where possible.