Gibco™ MEM, Hanks' Balanced Salts, 500 mL

Gibco™ Minimum Essential Medium (MEM) is one of the most widely used classical media for mammalian cell culture. Developed from the original formulation created by Harry Eagle, MEM supports the robust growth of many common suspension and adherent cell lines and remains a foundational medium in research laboratories worldwide.

This formulation contains Hanks’ Balanced Salts (HBSS), enabling its use in environments without CO₂ buffering, such as open vessels, transport conditions, or workflows outside CO₂ incubators. It includes L-glutamine and phenol red, but does not contain HEPES or sodium pyruvate.

Suitable for a wide range of mammalian cells—including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes—this medium is manufactured under ISO 13485 cGMP-compliant processes to ensure high quality, consistency, and reliability for research and further manufacturing applications.

Key Features

• Classical MEM formulation suitable for broad cell culture applications.
• Contains Hanks’ salts, allowing use without CO₂ incubation.
• Supplemented with L-glutamine and phenol red indicator.
• Sterile-filtered, animal origin–free, and produced under cGMP standards.
• Supports a wide variety of established cell lines and primary cell cultures.
• Shipped at ambient temperature; stored at 2–8°C, protected from light.

Formulation Overview

With:

• Hanks’ Balanced Salts (HBSS)
• L-glutamine
• Phenol red

Without:

• HEPES
• Sodium pyruvate

(A complete formulation is available upon request.)

Applications

This MEM formulation is ideal for:

• Routine culture and maintenance of mammalian cell lines
• Expansion of suspension and adherent cells
• Transfection workflows
• Assay development and screening
• Primary astrocyte cultures
• General research and upstream processing environments

Specifications

Gibco™ MEM, Hanks’ Balanced Salts provides a reliable, consistent, and versatile solution for laboratories working with a wide range of mammalian cell types. Its compatibility with both primary and established cell lines, combined with high-quality manufacturing and a formulation designed for use outside CO₂ incubators, makes it a dependable medium for daily research, cell expansion, and assay development. Whether used in academic, industrial, or bioprocessing labs, this MEM formulation delivers performance and stability that researchers trust.