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Specifications:
| Application | molecular biology, Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₃₂H₅₈N₂O₇S |
Proteomic-Grade Zwitterionic Detergent for IEF Fractionation and 2D Electrophoresis
Invitrogen™ ZOOM™ CHAPS is a high-purity, zwitterionic detergent supplied as part of the ZOOM™ IEF Fractionation system and designed specifically for sample solubilization in 2D electrophoresis and solution-phase isoelectric focusing (IEF). It is optimized for use with the ZOOM™ IEF Fractionator, providing excellent performance in the extraction, solubilization, and fractionation of complex protein samples from a wide variety of biological sources.
CHAPS is a preferred detergent for proteomic applications due to its ability to solubilize hydrophobic and membrane proteins while maintaining native protein conformation. This makes it ideal for non-denaturing protein extraction, especially in workflows involving 2D PAGE, LC/MS, and IEF-based fractionation.
Key Features & Benefits
- Proteomic-Grade Purity – Suitable for high-resolution 2D electrophoresis and mass spectrometry
- Zwitterionic and Non-Denaturing – Maintains protein structure while efficiently solubilizing proteins
- Compatible with IEF – Ideal detergent for sample preparation prior to solution-phase IEF or gel-based 2D PAGE
- Reliable Solubilization – Enhances resolution by improving the solubility of low-abundance and membrane proteins
- Part of the ZOOM™ Workflow – Optimized for use with the ZOOM™ IEF Fractionator and ZOOM™ 2D Protein Solubilizers
Applications
- 2D Gel Electrophoresis (2D-PAGE) – For high-resolution separation of complex protein mixtures
- Solution-Phase Isoelectric Focusing – Supports reproducible sample fractionation using ZOOM™ Disks
- Proteomics Sample Prep – Prepares samples for 2D LC/MS and IPG strip loading
- Membrane Protein Solubilization – Extracts hydrophobic proteins while preserving activity
- Basic Protein Fractionation – Compatible with basic protein kits in the ZOOM™ product line
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | ZOOM™ CHAPS |
| Catalog Number | ZC10003 |
| Format | Powder |
| Purity | Proteomic grade (≥98%) |
| Molecular Weight | ~614.88 g/mol |
| CMC (Typical) | 6–10 mM |
| Micelle Molecular Weight | ~6,150 g/mol |
| Aggregation Number | 4–14 |
| Dialyzable | Yes |
| Quantity | 5 g |
| Storage Conditions | Store in a dry, cool place (refrigerate after reconstitution) |
| Shipping Conditions | Room temperature |
| Unit Size | 5 g |
| Regulatory Use | For Research Use Only |
Invitrogen™ ZOOM™ CHAPS is a trusted component of the ZOOM™ IEF workflow, offering efficient, non-denaturing protein solubilization for isoelectric focusing and 2D proteomic analysis. Its proteomic-grade quality, excellent compatibility with ZOOM™ reagents, and reproducible performance make it an essential tool for researchers conducting high-resolution protein separations and complex sample analyses.