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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Non-Ionic Detergent for Laboratory and Research Applications
The Invitrogen™ Triton X-100 (1%) is a non-ionic detergent supplied as a ready-to-use 1% solution, primarily used in Fetal Hemoglobin (HbF) tests and various biochemical and molecular biology applications. It can be further diluted in phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and sodium azide to prepare a 0.1% working solution, making it ideal for cell permeabilization, protein extraction, and immunoassays.
For Research Use Only. Not for use in diagnostic procedures.
Key Features & Benefits
✅ Ready-to-Use 1% Solution
- Pre-diluted for convenience and consistency in laboratory workflows.
- Can be easily diluted to 0.1% for use in Fetal Hemoglobin (HbF) testing.
✅ Non-Ionic Detergent for Gentle Cell Permeabilization
- Suitable for membrane solubilization without protein denaturation.
- Ideal for immunocytochemistry (ICC), immunofluorescence (IF), and western blotting.
✅ Versatile Use in Molecular & Cell Biology Applications
- Compatible with flow cytometry, ELISA, and immunoassays.
- Useful in protein extraction, solubilization, and washing steps.
✅ Stable & Long Shelf Life
- Shelf life of 24 months from the date of manufacture.
- Refrigeration storage (2–8°C) ensures long-term stability.
Technical Specifications
| Feature | Details |
|---|---|
| Product Type | Detergent |
| Reagent Type | Triton X-100 (1%) |
| Form | Liquid |
| Quantity | 10 mL |
| Unit Size | Each |
| Storage Conditions | 2–8°C (Refrigerated) |
| Shelf Life | 24 months from the date of manufacture |
| Shipping Condition | Wet Ice |
| Dilution Recommendations | Dilute in PBS containing BSA and sodium azide for a 0.1% working solution |
| Catalog Number | HFH10 |
Use Cases & Applications
1. Fetal Hemoglobin (HbF) Testing
Primary Use:
- Used as a component in Fetal Hemoglobin (HbF) tests, where it is diluted to 0.1% working concentration.
Practical Example:
A clinical research laboratory investigating sickle cell disease and beta-thalassemia uses Invitrogen Triton X-100 (1%) in HbF staining protocols to differentiate fetal and adult hemoglobin in blood samples.
2. Cell Permeabilization for Immunofluorescence & Flow Cytometry
Primary Use:
- Used in flow cytometry (FACS) and immunofluorescence (IF) staining protocols for intracellular protein detection.
- Gently permeabilizes cell membranes, allowing antibody penetration without disrupting cell structure.
Practical Example:
A cancer research lab studying intracellular signaling pathways uses Triton X-100 in flow cytometry to permeabilize leukemia cell lines, enabling antibody staining of phosphorylated proteins.
3. Western Blotting & Protein Extraction
Primary Use:
- Acts as a detergent for protein extraction and solubilization.
- Used in lysis buffers to isolate membrane proteins and cytosolic proteins.
Practical Example:
A neuroscience lab studying Alzheimer’s disease biomarkers extracts tau and beta-amyloid proteins from brain tissue samples using a Triton X-100-containing lysis buffer, ensuring efficient protein solubilization.
4. ELISA & Immunoassays
Primary Use:
- Used in washing buffers for ELISA and immunoprecipitation assays.
- Helps reduce nonspecific binding and improve assay sensitivity.
Practical Example:
A biotech company developing monoclonal antibodies uses Triton X-100 in ELISA wash buffers to remove unbound antibodies, enhancing signal specificity in antibody-antigen detection assays.
5. Lipid & Membrane Research
Primary Use:
- Used for membrane solubilization studies in lipidomics and proteomics.
- Essential for lipid raft isolation and detergent-resistant membrane studies.
Practical Example:
A cell biology lab analyzing cholesterol-enriched lipid rafts in T-cell signaling uses Triton X-100 to selectively solubilize membrane proteins, ensuring accurate fractionation of detergent-resistant and detergent-soluble components.
Ordering Information
| Catalog Number | Description | Quantity |
|---|---|---|
| HFH10 | Triton X-100 (1%) | 10 mL |
Why Choose Invitrogen™ Triton X-100 (1%)?
🧪 Pre-Diluted for Convenience – 1% solution eliminates the need for weighing and dissolving solid Triton X-100.
🔬 Compatible with Molecular & Cell Biology Applications – Ideal for protein extraction, immunoassays, and microscopy.
💧 Gentle Yet Effective Permeabilization – Maintains cell integrity while allowing intracellular antibody access.
⚡ Stable & Reliable – Long shelf life (24 months) with refrigerated storage (2–8°C) for consistent results.
🛡 High-Quality Research Reagent – Manufactured under strict quality control to ensure reproducibility in experiments.
The Invitrogen™ Triton X-100 (1%) is a high-quality, research-grade detergent optimized for cell permeabilization, protein extraction, immunoassays, and fetal hemoglobin testing. Its ready-to-use format, reliable performance, and versatility make it an essential reagent for biochemistry, molecular biology, and diagnostic research.