VWR Dithiothreitol (DTT, Cleland's reagent) ≥98%

VWR Dithiothreitol (DTT, also known as Cleland's reagent) is a high-purity (≥98%) reducing agent commonly used in biochemical, molecular biology, and proteomics applications. DTT is a water-soluble thiol-containing compound that acts as a potent disulfide bond breaker, preventing oxidation of proteins, enzymes, and nucleic acids.

This reducing agent is widely used in protein electrophoresis (SDS-PAGE), enzyme activity preservation, thiol modification studies, and RNA/DNA stabilization. It is also essential in reducing disulfide bonds in proteins before mass spectrometry, Western blotting, and structural analysis.

DTT is a small-molecule thiol (-SH) compound that provides a strong reducing environment and is highly effective in maintaining proteins and other biological molecules in their active, reduced state.

Key Features & Benefits

1. High-Purity Reducing Agent (≥98%)
   • Ensures consistent and reproducible results in biochemical and molecular biology experiments.
   • Provides efficient reduction of protein disulfide bonds in proteomics research.
2. Prevents Oxidation in Proteins, Enzymes & Nucleic Acids
   • Maintains enzyme stability by preventing oxidation of cysteine residues.
   • Protects RNA and DNA samples from oxidative degradation.
3. Strong Reducing Agent for Protein Analysis & Structural Studies
   • Essential in SDS-PAGE sample preparation for reducing protein disulfide bonds.
   • Used in protein refolding, mass spectrometry, and Western blotting.
4. Compatible with Various Biological Buffers & Solutions
   • Easily dissolves in Tris, PBS, and other biological buffers.
   • Maintains protein activity and solubility in experimental conditions.
5. Versatile Applications in Biochemistry & Molecular Biology
   • Used in protein purification, thiol modification, enzyme activity assays, and genetic studies.
   • Acts as a powerful reducing agent in redox-based cellular and biochemical research.

Product Specifications

Use Cases & Applications

1. Protein Analysis & Proteomics

• Reduction of Disulfide Bonds in Proteins for SDS-PAGE
  • Ensures proper protein denaturation for electrophoresis analysis.
  • Prevents disulfide-mediated aggregation of proteins in gel electrophoresis.
• Western Blotting & Mass Spectrometry Sample Preparation
  • Used to reduce disulfide bonds in proteins before immunodetection.
  • Improves peptide fragmentation in mass spectrometry-based proteomics.

2. Enzyme Stability & Thiol Modification Studies

• Maintains Enzyme Activity in Biochemical Reactions
  • Prevents oxidation of thiol (-SH) groups in active enzyme sites.
  • Used in redox regulation and enzymatic assays requiring reducing conditions.
• Thiol Modification & Structural Biology Research
  • Essential for studying protein folding and thiol-mediated interactions.
  • Used in X-ray crystallography and nuclear magnetic resonance (NMR) studies.

3. Molecular Biology & RNA/DNA Research

• Protects RNA from Oxidation in Reverse Transcription & cDNA Synthesis
  • Prevents RNA degradation due to oxidation.
  • Used in RT-PCR, qPCR, and mRNA isolation workflows.
• Enhances DNA Stability in Genetic Studies
  • Maintains integrity of genomic and plasmid DNA in biochemical experiments.
  • Ensures high-quality DNA for cloning, sequencing, and PCR applications.

4. Biochemical & Cellular Redox Research

• Maintains Reducing Conditions in Redox-Sensitive Experiments
  • Acts as a cell-permeable reducing agent for in vitro assays.
  • Used in cell culture experiments to prevent oxidative stress in proteins.
• Redox Control in Cellular Signaling Pathways
  • Investigates cellular oxidative stress and thiol-based protein modifications.
  • Plays a role in disulfide-mediated protein regulation.

Storage & Handling Instructions

• Storage Conditions:
  • Store at -20°C in a dry, airtight container.
  • Avoid prolonged exposure to air and moisture to prevent oxidation.
• Handling Precautions:
  • Use gloves, safety goggles, and a lab coat when handling.
  • Prepare fresh DTT solutions before use, as it is sensitive to oxidation.
  • Work in a well-ventilated area to prevent exposure to reducing vapors.
• Disposal Guidelines:
  • Dispose of according to local chemical waste regulations.
  • Avoid releasing into sewer systems or natural water bodies.

VWR DTT is a trusted, high-quality reducing agent designed for biochemistry, proteomics, molecular biology, and enzymatic research, making it an indispensable tool for laboratories and scientific institutions.