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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreen)
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Reading Options: Top and Bottom
No. of dispensers: 0
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader is an advanced, modular, and user-friendly instrument designed for life-science and drug-discovery laboratories requiring high-performance multimode detection. It combines up to five measurement technologies—Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence (TRF), and AlphaScreen/AlphaLISA—within a single compact system.
Featuring automatic dynamic range selection, spectral scanning, and smart safety controls, Varioskan™ LUX ensures reliability, reproducibility, and ease of use across a wide range of microplate-based assays.
Key Features
- Five integrated detection modes: Absorbance (UV-Vis), Fluorescence Intensity (including FRET), Luminescence (direct and filtered), AlphaScreen/AlphaLISA, and Time-Resolved Fluorescence (including TR-FRET/hTRF).
- Filter + Monochromator Optics: Flexibility for assay optimization and spectral scanning.
- Automatic Dynamic Range Selection: Eliminates the need for manual gain adjustment.
- Smart Safety Controls: Proactive system diagnostics prevent user errors and instrument damage.
- Integrated Gas Control Module: Enables CO₂ and O₂ regulation for cell-based assays.
- Simultaneous Dispensing and Measurement: Captures kinetic signals from the very start of a reaction.
- HTRF™ Certified (Cisbio Bioassays) and DLReady™ Validated (Promega Corporation) for trusted assay compatibility.
- Modular and Upgradeable: Expand measurement capabilities as laboratory needs evolve.
- Powerful SkanIt™ Software: Intuitive, license-free software for assay setup, data acquisition, and analysis.
Software Highlights (SkanIt™ PC Software, Research Edition)
- License-free installation for multiple users.
- Virtual Pipetting Tool for easy plate layout design.
- Real-time visualization of spectra and kinetic curves.
- Continuous autosave to prevent data loss from interruptions.
- Automated data export to *.xlsx, *.pdf, *.xml, or *.txt formats.
- Comprehensive calculations: blank subtraction, averages, SD/CV%, standard/dose-response curves, quality control, and custom formulas.
- Detailed result reports with full traceability and audit trail.
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Two editions available:
- Research Edition (included)
- Drug Discovery Edition (for FDA 21 CFR Part 11 compliance).
Applications
- Cell-based and biochemical assays
- FRET/TR-FRET, AlphaScreen/AlphaLISA detection
- Reporter gene and ATP-based luminescence assays
- ELISA and nucleic-acid quantification
- Kinetic and spectral analysis
- Enzyme activity and inhibitor screening
- Fluorescent dye or protein quantification
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreen |
| Reading Options | Top and Bottom |
| Wavelength Range | Absorbance 200–1000 nm; Fluorescence Exc. 200–822 nm, Em. 270–840 nm; TRF Exc. 334 nm, Em. 400–700 nm; Luminescence 360–670 nm; AlphaScreen Exc. 680 nm, Em. 400–660 nm |
| Measurement Modes | Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra |
| Spectral Scanning Speed | < 2.2 s/well (400–500 nm, 1 flash, 2 nm step) |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI Top > 6 decades; FI Bottom > 5.5 decades; Luminescence > 7 decades |
| Sensitivity | FI Top < 0.4 fmol fluorescein/well; FI Bottom < 4 fmol; TRF < 1 amol/well; Luminescence < 7 amol ATP/well; AlphaScreen < 100 amol/well |
| Precision | SD < 0.001 Abs or CV < 0.5% (at 450 nm, 0–3 Abs) |
| Accuracy (Photometric) | 0.003 Abs or ±2% (200–399 nm), ±1% (400–1000 nm) |
| Plate Types | 6 to 1536-well plates (Absorbance 6–384-well), μDrop plates |
| Incubator Temperature | Ambient + 4 °C to 45 °C |
| Shaking | Orbital |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp, LED |
| Dimensions (L×W×H) | 58 × 53 × 51 cm (23 × 21 × 20 in.) |
| Weight | 57 kg (125 lb) |
| Interface | Thermo Scientific™ SkanIt™ Software |
| Product Line | Varioskan™ |
| Catalog Number | VLBLATD0 |
| Includes | SkanIt™ PC Software (Research Edition), Nunc™ 96-Well Optical-Bottom Microplate (black, TC surface) |
Unique Advantages
- High versatility for diverse assay formats and plate types (6 – 1536 wells).
- Exceptional sensitivity and linearity, ensuring robust detection from low to high signal ranges.
- Expandable configuration protects long-term investment.
- Validated for regulatory and high-throughput environments with optional Drug Discovery Edition compliance features.
Ordering Information
| Catalog Number | Measurement Technologies | Reading Options | No. of Dispensers | Includes |
|---|---|---|---|---|
| VLBLATD0 | Absorbance, Fluorescence Intensity, Luminescence, TRF, AlphaScreen | Top and Bottom | None | SkanIt PC Software (Research Edition) |
The Varioskan™ LUX Multimode Microplate Reader is a robust, future-ready solution for academic, biotechnology, and pharmaceutical laboratories. Its modularity, precision, and intelligent automation make it ideal for demanding workflows that require flexibility, reliability, and regulatory-ready performance.