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Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreen)
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Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TGD2) is a versatile and modular detection platform that delivers exceptional sensitivity and reliability for a wide range of biochemical and cell-based assays.
This configuration combines four core detection technologies—Absorbance (UV-Vis), Fluorescence Intensity, Luminescence, and Time-Resolved Fluorescence (TRF)—with an integrated Gas Control Module and two independent reagent dispensers, enabling simultaneous measurement and real-time reagent addition during kinetic assays.
Built for advanced applications in cell biology, enzymology, and molecular diagnostics, the Varioskan™ LUX provides automatic dynamic range selection, spectral scanning, and smart safety controls that streamline workflows and ensure data integrity.
Key Features
- Four measurement technologies: Absorbance (UV-Vis), Fluorescence Intensity (including FRET), Luminescence, and Time-Resolved Fluorescence (TR-FRET/hTRF).
- Integrated Gas Control Module: Regulates CO₂ (0.1–15 %) and O₂ (1–21 %) for live-cell, metabolic, and hypoxia studies.
- Dual Dispensers: Two independent liquid-handling channels (2 – 5 000 µL, 1 µL increments) for sequential or simultaneous reagent addition during kinetic runs.
- Automatic Dynamic Range Selection: Optimizes signal intensity per well—eliminating manual gain adjustments.
- High-speed spectral scanning for assay optimization (< 2.2 s/well, 2 nm step).
- Smart Safety Controls and Self-Diagnostics prevent user and mechanical errors.
- HTRF™ certified by Cisbio and DLReady™ validated by Promega for assay compatibility.
- Automation-ready: Integrates seamlessly with robotic plate handlers for high-throughput workflows.
- Upgradeable architecture: Expandable to include additional modules or new detection capabilities.
SkanIt™ Software (Research Edition Included)
- License-free use: Each user may install on their own computer.
- Virtual Pipetting Tool for quick sample-to-plate layout definition.
- Real-time data display of kinetic and spectral readings.
- Automatic data saving prevents loss during power or software interruptions.
- Advanced analysis tools: blank subtraction, SD/CV %, standard curves, dose responses, kinetic and spectral evaluation, and custom calculations.
- Flexible data export: *.xlsx, *.pdf, *.xml, *.txt or automatic email delivery.
- Audit-ready reporting for traceable records.
- Optional Drug Discovery Edition supports FDA 21 CFR Part 11 compliance.
Applications
- Cell-based assays requiring CO₂/O₂ regulation
- Reporter gene and luminescence assays
- Enzyme kinetics and real-time signal monitoring
- TR-FRET (hTRF) interaction studies
- Spectral scanning and assay optimization
- Protein and nucleic acid quantification
- Drug screening and cytotoxicity testing
Technical Specifications
| Parameter | Specification |
|---|---|
| Catalog Number | VLBL0TGD2 |
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence |
| Reading Options | Top and Bottom |
| Dispensers | 2 (2 – 5 000 µL, 1 µL increments) |
| Gas Control Module | CO₂ 0.1–15 %; O₂ 1–21 % |
| Measurement Modes | Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra |
| Spectral Scanning Speed | < 2.2 s/well (400–500 nm, 1 flash, 2 nm step) |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | FI > 6 decades; Lum > 7 decades; TRF > 6 decades |
| Sensitivity | FI (top) < 0.4 fmol fluorescein/well; Lum < 7 amol ATP/well; TRF < 1 amol/well |
| Photometric Accuracy | 0.003 Abs or ±2 % (200–399 nm, 0–2 Abs); ±1 % (400–1000 nm, 0–3 Abs) |
| Linearity | ±2 % (96-well 0–4 Abs @ 450 nm; 384-well 0–3 Abs) |
| Precision | SD < 0.001 Abs or CV < 0.5 % (450 nm, 0–3 Abs) |
| Wavelength Range | Abs 200–1000 nm; FI Exc. 200–822 nm / Em. 270–840 nm; TRF Exc. 334 nm / Em. 400–700 nm; Lum 360–670 nm |
| Incubator Temperature | Ambient + 4 °C to 45 °C |
| Plate Compatibility | 6–1536 wells (Abs 6–384 wells); μDrop plates |
| Detector Type | Photomultiplier Tube (PMT) |
| Light Source | Xenon Flash Lamp |
| Shaking | Orbital |
| Dimensions (L × W × H) | 58 × 53 × 51 cm (23 × 21 × 20 in.) |
| Weight | 57 kg (125 lb) |
| Interface | Thermo Scientific™ SkanIt™ Software |
| Read-out Range | 0 – 6 Abs |
| Product Line | Varioskan™ |
| Included Accessories | Gas Module, Dual Dispensers, SkanIt PC Software (Research Edition), Nunc™ 96-Well Optical-Bottom Microplate (black, TC surface) |
Unique Advantages
- Dual dispensers enable rapid, programmable reagent addition for kinetic and enzyme assays.
- Integrated gas control ensures physiological assay conditions for cell-based research.
- High-sensitivity optics deliver fmol–amol detection levels across multiple technologies.
- Smart automation and diagnostics provide reliability and ease of use.
- Upgradeable platform supporting future expansion and automation integration.
Ordering Information
| Catalog Number | Measurement Technologies | Reading Options | Dispensers | Includes |
|---|---|---|---|---|
| VLBL0TGD2 | Absorbance, Fluorescence Intensity, Luminescence, Time-Resolved Fluorescence | Top and Bottom | 2 | Gas Module and SkanIt PC Software (Research Edition) |
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL0TGD2) is a high-end multimode instrument combining precision optics, dual dispensers, and gas control in one fully automated system. Designed for demanding laboratory applications—from ELISA and kinetic studies to live-cell and luminescence assays—it delivers unrivaled sensitivity, speed, and operational confidence.