0
Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity, Luminescence)
Catalog No :
CAS Number :
Brand :
In Stock
Reading Options: Top and bottom
No. of dispensers: 1
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00D1) provides advanced, flexible, and precise multimode detection for absorbance, fluorescence intensity, and luminescence assays.
Equipped with a single high-precision dispenser, the Varioskan LUX enables simultaneous reagent addition and signal measurement, allowing kinetic assays to start immediately after reagent dispensing—ideal for enzyme kinetics, luminescence-based reactions, and other time-sensitive experiments.
Designed for reliability, efficiency, and accuracy, the instrument integrates smart automation, dynamic range selection, and self-calibration, ensuring reproducible, high-quality results for a wide range of applications in life sciences, molecular biology, and drug discovery.
Key Features and Benefits
1. Versatile Multimode Detection
- Combines Absorbance (UV-Vis, 200–1000 nm), Fluorescence Intensity, and Luminescence detection technologies.
- Top and bottom reading optics enable flexibility for both homogeneous and cell-based assays.
- Dual monochromator system provides precision wavelength selection for excitation and emission.
- Spectral scanning supports full-spectrum optimization for assay development.
- Compatible with 6–1536 well microplates and μDrop plates for small-volume assays.
2. Integrated Dispensing System
- One built-in dispenser (2–5,000 μL, 1 μL increments) for accurate reagent addition.
- Enables simultaneous dispensing and measurement—ideal for kinetic and enzyme-linked assays.
- Reduces manual pipetting errors and improves workflow efficiency.
3. High Sensitivity and Dynamic Range
-
Fluorescence Sensitivity:
- Top read: <0.4 fmol fluorescein/well
- Bottom read: <4 fmol fluorescein/well
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
-
Dynamic Range:
- Fluorescence: >6 decades (top), >5.5 decades (bottom)
- Luminescence: >7 decades
- Photometric Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm (0–3 Abs).
4. Intelligent Automation and Workflow Control
- Automatic dynamic range selection optimizes signal detection per well.
- Smart safety controls alert users to setup or operational errors.
- Automated self-calibration and diagnostics ensure stable, reproducible performance.
- Five measurement modes: Endpoint, kinetic, spectra, kinetic spectra, and multipoint.
- Temperature control (ambient +4°C to 45°C) and orbital shaking maintain homogeneous reactions.
5. SkanIt™ Software – Intuitive Data Management
- SkanIt™ Research Edition Software included with the system.
- License-free installation—accessible to all users in a lab.
- Virtual Pipetting Tool streamlines plate setup and assay mapping.
- Real-time kinetic and spectral data display during runs.
- Automatic data backup prevents data loss during interruptions.
- Built-in data analysis: Blank subtraction, standard curve, dose-response, kinetic, and custom formulae.
- Export flexibility: *.xlsx, *.pdf, *.xml, *.txt formats with auto-email function post-run.
6. Certified and Upgradeable
- HTRF™ certified by Cisbio Bioassays for homogenous time-resolved assays.
- DLReady™ validated by Promega Corporation for luminescence applications.
- Upgradeable modular design allows addition of TRF or AlphaScreen™ technologies.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence |
| Reading Options | Top and Bottom |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; Luminescence: 360–670 nm |
| Dispensing Volume | 2–5,000 μL (1 μL increments) |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence: Top >6 decades, Bottom >5.5 decades; Luminescence >7 decades |
| Sensitivity | FI Top: <0.4 fmol fluorescein/well; FI Bottom: <4 fmol fluorescein/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm), ±1% (400–1000 nm) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well: 0–4 Abs; 384-well: 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 1 flash, 2 nm step) |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Interface | PC Software (SkanIt™ Software) |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 57 kg (125 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Compatibility | 6–1536 well plates; μDrop plates |
| Software Edition | Research Edition |
Applications
- Enzyme Kinetics: Real-time monitoring of enzyme catalysis and inhibition.
- Cell-Based Assays: Reporter gene, viability, and cytotoxicity analysis.
- Fluorescence and Luminescence Assays: GFP/FRET assays, luciferase, and ATP detection.
- Protein and Nucleic Acid Quantification: DNA/RNA and protein measurement via absorbance.
- Drug Discovery: Kinetic luminescence screening and compound testing.
- Spectral Scanning: Excitation/emission optimization for assay development.
Unique Advantages
- Combines Absorbance, Fluorescence, and Luminescence in one compact platform.
- Integrated single dispenser enables immediate kinetic measurements.
- Top and bottom reading optics enhance versatility for various assay types.
- Automatic calibration and self-diagnostics ensure accurate, reproducible data.
- HTRF™ and DLReady™ certified for validated performance.
- Modular system design allows future technology upgrades.
- Includes SkanIt™ Software for traceable, multi-user data analysis and reporting.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00D1) combines three core detection technologies—Absorbance, Fluorescence, and Luminescence—with a built-in dispenser for real-time reaction monitoring and kinetic measurements.
With automatic calibration, dual optics (top and bottom reading), and powerful SkanIt™ Software, it provides unparalleled accuracy, flexibility, and ease of use.
Designed for research laboratories, pharmaceutical R&D, and biotechnology facilities, the Varioskan LUX enables advanced multimode assay workflows with high throughput and reproducibility.