Varioskan™ LUX multimode microplate reader (Absorbance, Fluorescence Intensity)

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Reading Options: Top and bottom
No. of dispensers: None

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Specifications:

Application	molecular biology	Warranty	1 Year
Storage Temperature	Ambient
Product Type	Laboratory Equipment
Product Brand	Thermo Fisher Scientific™
Product Grade	Molecular Biology

Overview
Resource

The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLB000D0) is an advanced and reliable dual-optics microplate reader designed for bioscience, pharmaceutical, and life science researchers requiring flexible detection for absorbance and fluorescence assays.

With both top and bottom reading modes, the Varioskan LUX ensures optimal signal detection across a wide range of assays, including protein quantification, enzyme kinetics, fluorescence-based cellular assays, and DNA/RNA analysis.

This compact, modular system features automatic dynamic range selection, smart safety controls, and an intuitive SkanIt™ Software interface, streamlining data acquisition and minimizing user error while ensuring reproducible results every time.

Key Features and Benefits

1. Dual Detection and Reading Flexibility

Absorbance (UV-Vis) and Fluorescence Intensity detection in one instrument.
Top and bottom reading options optimize detection for cell-based and solution assays.
Double monochromator optics for precise wavelength selection and assay versatility.
Spectral scanning for rapid assay optimization and wavelength characterization.
Compatible with 6–1536 well plates and μDrop plates for small-volume assays.

2. Performance and Efficiency

High sensitivity:
- Top fluorescence reading: <0.4 fmol fluorescein/well.
- Bottom fluorescence reading: <4 fmol fluorescein/well.
Fast measurement speed: 15 s (96-well), 45 s (384-well), and 135 s (1536-well).
Dynamic range: >6 decades (top) and >5.5 decades (bottom).
Precision: SD <0.001 Abs or CV <0.5% at 450 nm.
Photometric accuracy: ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs).
Linearity: ±2% across 0–4 Abs (96-well) and 0–3 Abs (384-well).

3. Smart Automation and Safety

Automatic dynamic range selection per well ensures optimal signal detection.
Automated calibration and self-diagnostics guarantee long-term accuracy.
Smart safety controls alert users to potential issues before they occur.
Orbital shaking and temperature control (ambient +4°C to 45°C) maintain assay consistency.

4. Powerful SkanIt™ Software

SkanIt™ Research Edition included, with a license-free multi-user installation.
Virtual Pipetting Tool for quick and accurate plate setup.
Real-time spectral and kinetic data visualization.
Automatic data saving prevents loss from unexpected interruptions.
Built-in analysis tools: Blank subtraction, standard curve, dose-response, kinetic, and spectral analysis.
Flexible export options: *.xlsx, *.pdf, *.xml, and *.txt file formats.
Traceable reports with full runtime action documentation.

5. Certified and Upgradeable

HTRF™ certified by Cisbio Bioassays.
DLReady™ validated by Promega Corporation.
Modular, upgradeable platform for future addition of luminescence, AlphaScreen™, and time-resolved fluorescence technologies.

Technical Specifications

Parameter	Specification
Measurement Technologies	Absorbance, Fluorescence Intensity
Reading Options	Top and Bottom
Wavelength Range	Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm
Measurement Speed	15 s (96-well), 45 s (384-well), 135 s (1536-well)
Dynamic Range	Fluorescence Intensity: Top >6 decades, Bottom >5.5 decades
Sensitivity	<0.4 fmol fluorescein/well (top), <4 fmol fluorescein/well (bottom)
Accuracy (Photometric)	±2% (200–399 nm), ±1% (400–1000 nm)
Precision	SD <0.001 Abs or CV <0.5% at 450 nm
Linearity	±2% (96-well 0–4 Abs; 384-well 0–3 Abs at 450 nm)
Spectral Scanning Speed	<2.2 s/well (400–500 nm, 1 flash, 2 nm step)
Light Source	Xenon Flash Lamp
Detector Type	Photomultiplier Tube (PMT)
Incubation Temperature	Ambient +4°C to 45°C
Shaking	Orbital
Interface	PC Software (SkanIt™ Software)
Software Languages	English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese
Dimensions (W × D × H)	53 × 58 × 51 cm (21 × 23 × 20 in)
Weight	54 kg (119 lb)
Power Requirements	100–240 V, 50/60 Hz
Plate Compatibility	6–1536 well plates; μDrop plates
Software Type	SkanIt™ PC Software, Research Edition

Applications

Absorbance Assays: Nucleic acid (DNA/RNA) and protein quantification.
Fluorescence Assays: FRET, GFP, and fluorophore-based molecular interaction studies.
Enzyme Kinetics: Reaction rate and inhibition analysis.
Protein and Biomolecule Analysis: BCA, Bradford, and Lowry assays.
Cell-Based Assays: Reporter gene expression, cell proliferation, and cytotoxicity.
Spectral Scanning: Optimization of excitation/emission wavelengths.
High-Throughput Screening: Multi-well kinetic and endpoint analysis.

Unique Advantages

Combines absorbance and fluorescence detection with both top and bottom reading options.
High sensitivity and wide dynamic range for low-signal assays.
Smart safety and calibration systems ensure reproducibility.
Seamless data management and traceability with SkanIt™ Software.
Modular, upgradable design for future technology integration.
Validated and certified performance (HTRF™, DLReady™).
Compact, robust construction for continuous, reliable operation in research labs.

The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLB000D0) combines absorbance and fluorescence measurement technologies with dual top and bottom reading optics for comprehensive assay flexibility.

Engineered for precision, reliability, and ease of use, this modular platform supports spectral scanning, kinetic, and multipoint measurements—making it an ideal solution for life science research, molecular biology, and drug discovery applications.

Paired with SkanIt™ Software (Research Edition), the Varioskan LUX ensures superior data integrity, real-time monitoring, and seamless analysis, delivering powerful results for every laboratory workflow.